pCMV-HA-chip Plasmid

Quantity:

Properties

Precipitation of Chromatins is a pull down technique of native DNA sequences by specific binding proteins on which on the other side of the protein the antibody can bind. Lots of antibodies are not suited for Chip analysis pulldown because they bind on the binding site of the DNA. The Lifescience Market antibody is proven to not bind on the DNA binding site and is suitable for ChiP precipitation supplied in 1 vials.
Kit

Plasmid mini made and maxi DNA purification kits can be silica gel or anion exchange, endotoxin free and are used to produce pure plasmids that are small DNA molecules within a cell separated from chromosomal DNA and can replicate independently. They are most commonly found in bacteria as small circular, double-stranded DNA molecules; however, plasmids are sometimes present in archaea and eukaryotic organisms. In nature, plasmids often carry genes that may benefit the survival of the organism, for example antibiotic resistance. While the chromosomes are big and contain all the essential information for living, plasmids usually are very small and contain only additional information. Artificial plasmids are widely used as vectors in molecular cloning, serving to drive the replication of recombinant DNA sequences within host organisms.

Gene target

pCMV-HA
Gene symbol

MYO1G, KRT31, KRT32, KRT33B, KRT35, KRT34, ARHGAP45, KRT33A, AKAP13, PUM3
Short name

pCMV-HA-chip Plasmid
Technique

plasmid, ChiP, Chromatine immuno Precipitationg antibody, plasmids in 1
Alternative name

pCMV-HA-chip Plasmid
Alternative technique

plasmids, epigenetics

Quantity:

Identity

HGNC:13880
Gene

MYO1G
Long gene name

myosin IG
Synonyms
- HA-2
Synonyms name
- minor histocompatibility antigen HA-2
GenBank acession
- AF380932
Locus

7p13
Discovery year

2000-11-28
Entrez gene record

64005
Classification
- Myosin heavy chains, class I
- Minor histocompatibility antigens
VEGA ID

OTTHUMG00000155821

Identity

HGNC:17102
Gene

ARHGAP45
Long gene name

Rho GTPase activating protein 45
Synonyms gene
- HMHA1
Synonyms gene name
- histocompatibility (minor) HA-1
Synonyms
- KIAA0223
- HA-1
GenBank acession
- D86976
Locus

19p13.3
Discovery year

2005-12-06
Entrez gene record

23526
Pubmed identfication
- 9820596
- 9039502
Classification
- Minor histocompatibility antigens
- AH/BAR family Rho GTPase activating proteins
- F-BAR domain containing

Identity

HGNC:371
Gene

AKAP13
Long gene name

A-kinase anchoring protein 13
Synonyms gene
- LBC
Synonyms gene name
- lymphoid blast crisis oncogene
- A kinase (PRKA) anchor protein 13
Synonyms
- Ht31
- BRX
- AKAP-Lbc
- c-lbc
- PROTO-LB
- HA-3
- ARHGEF13
GenBank acession
- M90360
Locus

15q25.3
Discovery year

1999-09-16
Entrez gene record

11214
Pubmed identfication
- 9627117
- 1860836
RefSeq identity
- NM_007200
Classification
- A-kinase anchoring proteins
- Dbl family Rho GEFs
- Minor histocompatibility antigens
- Pleckstrin homology domain containing
- MicroRNA protein coding host genes
VEGA ID

OTTHUMG00000172205

Identity

HGNC:29676
Gene

PUM3
Long gene name

pumilio RNA binding family member 3
Synonyms gene
- KIAA0020
Synonyms gene name
- KIAA0020
Synonyms
- XTP5
- PEN
- PUF6
- hPUF-A
- HA-8
- Puf-A
Synonyms name
- penguin homolog (Drosophila)
- minor histocompatibility antigen HA-8
GenBank acession
- AL832239
Locus

9p24.2
Discovery year

2004-01-06
Entrez gene record

9933
Pubmed identfication
- 7584026
- 7584028
- 21266351
RefSeq identity
- NM_014878
Classification
- Pumilio homology domain containing
- Minor histocompatibility antigens
VEGA ID

OTTHUMG00000019450