Alexa Fluor 488-conjugated Donkey Anti-Rabbit IgG (H+L) Secondary Antibody
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Catalog number
AS035
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Price
Please ask
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Size
100ul
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Antibody category
Secondary Antibody
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Gene name
IgG
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Clonality
Secondary Antibody
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Reactivity
none
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Conjugate
Alexa Fluor 488
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Applications
Immunofluorescence, Flow cytometry
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Ordering
To order the Alexa Fluor 488-conjugated Donkey Anti-Rabbit IgG (H+L) Secondary Antibody (Cat. ID#AS035), please submit a purchase order by fax or e-mail. Please contact us for bulk or larger quantity.
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Tips
Please follow carefully the instructions before every use. Check the vial for the expiry date and batch number. If you need technical assistance please do not hesitate to contact us.
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Notes
For Research Use Only, not for therapeutic or diagnostic purposes. Not for human or animal consumption.
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Datasheet
Please contact our technical support to request the latest datasheet, protocol, certificate of analysis, MSDS.
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Properties
For facs or microscopy Alexa 1 conjugate. Alexa Fluor 488 has the same range to that of fluorescein isothiocyanate (FITC), yet the -conjugated Anti-Rabbit IgG (H+L) Secondary Antibody has a very high photo stability. As a result of this photo stability, it has turned into an antibody for fluorescent microscopy and FACS FLOW cytometry. It is distinguished in the FL1 of a FACS-Calibur or FACScan. Also Alexa Fluor 488 is pH stable. If you buy Antibodies supplied by ABclonal they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Conjugation
Alexa Fluor
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Latin name
Equus africanus asinus, Oryctolagus cuniculus
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rabbits are used for polyclonal antibody production by ABclonal. Rabbit antibodies are very stable and can be stored for several days at room temperature. ABclonal adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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French translation
anticorps
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Gene target
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Short name
-conjugated Anti-Rabbit IgG (H+L) Secondary Antibody
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Technique
Antibody, Rabbit, anti-, anti, IgG, antibody to, antibodies, antibodies against human proteins, antibodies for, antibody Conjugates, IgGs
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Host
Donkey, Donkeys or Equus africanus asinus are often used as host species to raise antibodies. Even Equus ferus or horse antibodies are different than donkey antibodies since we know horse anti donkey IgGs. For immunohistochemistry it is useful to use an anti-donkey or horse block if your primary antibody is a donkey anti-human. We provide also anti-donkey as a species antibodies and ELISA kits. Rabbits
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Isotype
IgG, IgG
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Label
ALEXA FLUOR 488
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Species
Donkeys
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Alternative name
Alexa Fluor 488-coupled Donkey Antibody tohost: rabbit Immunoglobulin G (heavy + light chain) secondary detector Antibody (antibody to-)
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Alternative technique
antibodies, rabbit-anti, immunoglobulins
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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