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Alternative_name
FITC-Val-Asp-Val-Ala-Asp-fluoromethyl ketone; FITC-VD(OMe)-VAD(OMe)-FMK
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Description
FITC-VD(OMe)VAD(OMe)-FMK is a fluorescein isothiocyanate (FITC) conjugate of the cell- permeable caspase-2 inhibitor VD(OMe)VAD(OMe)-FMK that acts as an in situ marker for detection of caspase-2 in living cells. FITC-VD(OMe)VAD(OMe)-FMK is nontoxic, and irreversibly binds to activated caspase-2 in apoptotic cells. The bound marker is localized by fluorescence detection.
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Structure Available
Yes
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Peptide sequence
FITC-VD(OMe)-VAD(OMe)-FMK
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Salt Form
No
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Molecular Formula
C₄₅H₅₁FN₆O₁₄S
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Molecular Weight
950.98
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Cell Permeable
Yes
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Purity
≥98% by HPLC
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Handling
Protect from air and moisture
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Tag Line
A caspase-2 in situ marker
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Storage Condition
-20°C
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Shipping Condition
gel pack
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Shelf Life
36 months
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SMILES
O=C(OC)C[C@H](NC([C@@H](NC([C@@H](NC([C@@H](NC([C@@H](N/C(S)=N/C1=CC=C(C2(C3=CC=C(O)C=C3OC4=C2C=CC(O)=C4)OC5=O)C5=C1)C(C)C)=O)CC(OC)=O)=O)C(C)C)=O)C)=O)C(CF)=O
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InChi
InChI=1S/C45H51FN6O14S/c1-20(2)37(41(60)47-22(5)39(58)49-30(32(55)19-46)17-35(56)63-6)51-40(59)31(18-36(57)64-7)50-42(61)38(21(3)4)52-44(67)48-23-8-11-27-26(14-23)43(62)66-45(27)28-12-9-24(53)15-33(28)65-34-16-25(54)10-13-29(34)45/h8-16,20-22,30-31,37-38,53-54H,17-19H2,1-7H3,(H,47,60)(H,49,58)(H,50,61)(H,51,59)(H2,48,52,67)/t22-,30-,31-,37-,38-/m0/s1
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InChi Key
UDNJMPACZZTQQK-MZPOKIFTSA-N
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MSDS Available
Yes
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Properties
This Biovision Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Biovision FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
