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Synonyms
VLDLR, CARMQ1, CHRMQ1, FLJ35024, VLDLRCH, VLDL-R, very-low-density-lipoprotein receptor
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Alternative_names
VLDLR, CARMQ1, CHRMQ1, FLJ35024, VLDLRCH, VLDL-R, very-low-density-lipoprotein receptor
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Description
Plays a significant role in lipid metabolism and in nervous system development and function
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Recombinant
Yes
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Source
HEK293 cells
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Purity by SDS PAGE
≥97%
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Assay
SDS-PAGE
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Biological activity
Measured by its binding ability in a functional ELISA. When Recombinant Human Apolipoprotein E3 is immobilized at 1 ug/ml (100 ul/well), the concentration of Recombinant Human VLDLR that produces 50% of the optimal binding response is found to be approximately 0.03 - 0.15 ug/ml.
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Results
Measured by its binding ability in a functional ELISA. When Recombinant Human Apolipoprotein E3 is immobilized at 1 ug/ml (100 ul/well), the concentration of Recombinant Human VLDLR that produces 50% of the optimal binding response is found to be approximately 0.03 - 0.15 ug/ml.
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Molecular Weight
This protein comprises 781 amino acids with polyhistidine tag at C-terminus and has a calculated MW of 86 kDa. The predicted N-terminus is Gly 28. DTT-reduced protein migrates as 150 & 180 kDa polypeptide in SDS-PAGE due to different glycosylation.
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Storage Temp
-20°C
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Shipping
Gel Pack
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Shelf Life
12 months
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Appearance
Lyophilized
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Physical form description
Lyophilized from 0.22 um filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
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Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
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Background Information
The very-low-density-lipoprotein receptor (VLDL-R) is a lipoprotein receptor that shows considerable similarity to the low density-lipoprotein receptor. VLDL R is a 130 kDa type I transmembrane protein in the LDL receptor family that plays a significant role in lipid metabolism and in nervous system development and function .This receptor has been suggested to be important for the metabolism of apoprotein-E-containing triacylglycerol-rich lipoproteins, such as very-low-density lipoprotein (VLDL), beta-migrating VLDL and intermediate-density lipoprotein. It is also one of the receptors of reelin, an extracellular matrix protein which regulates the processes of neuronal migration and synaptic plasticity. In humans, the VLDL-R is encoded by the VLDLR gene. A rare neurological disorder first described in the 1970s under the name "disequilibrium syndrome" is now considered to be caused by the disruption of VLDLR gene. The disorder was renamed VLDLR-associated cerebellar hypoplasia (VLDLRCH) after a 2005 study. It is associated with parental consanguinity and found in secluded communities such as the Hutterites. VLDLRCH is one of the two known genetic disorders caused by a disruption of reelin signaling pathway, along with Norman-Roberts syndrome.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
VLDLR-AS1, VLDLR
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Short name
CellExp™ VLDLR, recombinant
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Species
Humans
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Alternative name
H. sapiens CellExp™ very low density lipoprotein receptor, H. sapiens Rec.
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Alternative technique
rec
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Alternative to gene target
very low density lipoprotein receptor, CARMQ1 and CHRMQ1 and VLDLRCH, VLDLR and IDBG-44752 and ENSG00000147852 and 7436, calcium-dependent protein binding, Extracellular, Vldlr and IDBG-155959 and ENSMUSG00000024924 and 22359, VLDLR and IDBG-631615 and ENSBTAG00000018517 and 282123
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