M-CSF, murine recombinant

Synonyms

Macrophage Colony Stimulating Factor, CSF-1, MGI-IM
Alternative_names

Macrophage Colony Stimulating Factor, CSF-1, MGI-IM, Lanimostim
Description

A hematopoietic growth factor
Recombinant

Yes
Source

E. coli
Purity by SDS PAGE

≥98%
Assay

SDS-PAGE
Purity

≥98%
Biological activity

The ED₅₀ as determined by the dose-dependent stimulation of the proliferation of murine M-NSF-60 cells is 1 x 10⁶ units/mg.
Molecular Weight

36.4 kDa
Storage Temp

-20°C
Shipping

Gel pack
Shelf Life

12 months
Appearance

Lyophilized protein
Physical form description

Sterile filtered and then lyophilized from 5 mM Tris, pH 8.0 + 100 mM NaCl.
Reconstitution Instructions

Reconstitute in H₂O to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other buffers and stored at 4°C for 1 week or –20°C for future use.
Background Information

Macrophage Colony stimulating Factor (M-CSF) is produced by various cell types, including fibroblasts, microphages, and endothelial cells. M-CSF is involved in both the development and function of monocytes/microphages. The recombinant M-CSF is a disulfide-linked homodimer composed of two 18.2 kDa subunits of 156 amino acid residues.
Handling

Centrifuge the vial prior to opening.
Usage

For Research Use Only! Not to be used in humans
Datasheet
Additional source

Recombinants or rec. proteins
Group

recombinants

Gene target

M-CSF murine
Gene symbol

CSF1, CPT1B, CSF2, IRAK3, PGAM2, CYP20A1, USP16, CYB5B, H3C14, CSF3
Short name

M-CSF, murine recombinant
Technique

Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
Alternative name

M-CSF, murine Rec.
Alternative technique

rec

Identity

HGNC:20503
Gene

H3C14
Long gene name

H3 clustered histone 14
Synonyms gene
- H3F2
- H3FM
- HIST2H3C
Synonyms gene name
- H3 histone family, member M
- histone 2, H3c
- histone cluster 2, H3c
- histone cluster 2 H3 family member c
Synonyms
- MGC9629
- H3/m
- H3
- H3.2
- H3/M
GenBank acession
- AF531307
Locus

1q21.2
Discovery year

2003-02-19
Entrez gene record

126961
Pubmed identfication
- 12408966
- 3013246
RefSeq identity
- NM_021059
Classification
- H3 histones
VEGA ID

OTTHUMG00000012190

Identity

HGNC:2438
Gene

CSF3
Long gene name

colony stimulating factor 3
Synonyms gene
- GCSF
- G-CSF
- C17orf33
Synonyms gene name
- chromosome 17 open reading frame 33
- colony stimulating factor 3 (granulocyte)
Synonyms
- MGC45931
Synonyms name
Locus

17q21.1
Discovery year

2001-06-22
Entrez gene record

1440
Pubmed identfication
- 3499671
- 3501046
RefSeq identity
- NM_172220
Classification
- Receptor ligands
VEGA ID

OTTHUMG00000133247

Name

Primary Cell Culture
Concept

Scope note: The initial culturing of cells derived directly from fresh TISSUES.
Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data