T4 DNA Ligase (5 u/ μl)

• Catalog number
  9101-250
• Price
  Please ask
• Size
  250 u

• Synonyms
  DNA Ligase
• Alternative_names
  T4 DNA Ligase
• Description
  An enzyme which anneals DNA strands together
• Recombinant
  Yes
• Source
  E. coli
• Assay
  SDS-PAGE
• Unit Definition
  One unit of T4 DNA ligase is the amount of enzyme activity required to ligate EcoR I digested 1.0 µg of plasmid DNA in 5 minutes at 20°C in a 20 µl Reaction.
• Storage Temp
  -80°C
• Shipping
  Dry ice
• Shelf Life
  12 months
• Concentration
  5 U/µl
• Appearance
  Liquid
• Physical form description
  ENZYME STORAGE BUFFER: 20 mM Tris-HCl, pH 7.5, 50 mM KCl, 0.1 mM EDTA, 5 mM DTT, 50% glycerol (v/v).
• Background Information
  T4 DNA ligase catalyzes the formation of phosphodiester bonds between 5’-phosphate and 3’-hydroxy ends of double-stranded DNA. Single stranded nicks in double-stranded DNA are also closed by T4 DNA ligase. T4 DNA ligase needs ATP as co-factor.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet

• Gene target
  DNA   Ligase  
• Gene symbol
  PRKDC, HLA-DOA
• Short name
  T4 DNA Ligase (5 u/ μl)
• Technique
  dna
• Alternative name
  T4 Desoxyribonucleic acid Ligase (5 u/ μl)

Gene info

• Identity
  HGNC:9413
• Gene
  PRKDC
• Long gene name
  protein kinase, DNA-activated, catalytic subunit
• Synonyms gene
  • HYRC
  • HYRC1
• Synonyms gene name
  • protein kinase, DNA-activated, catalytic polypeptide
• Synonyms
  • DNPK1
  • p350
  • DNAPK
  • XRCC7
  • DNA-PKcs
  • DNAPKc
  • DNA-PKC
  • p460
• Synonyms name
  • DNA-dependent protein kinase catalytic subunit
• Locus
  8q11.21
• Discovery year
  1993-11-09
• Entrez gene record
  5591
• Pubmed identfication
  • 7638222
• RefSeq identity
  • NM_001081640
• Classification
  • Armadillo like helical domain containing
• VEGA ID
  OTTHUMG00000164239
• Locus Specific Databases
  • LRG_162

Gene info

• Identity
  HGNC:4936
• Gene
  HLA-DOA
• Long gene name
  major histocompatibility complex, class II, DO alpha
• Synonyms gene
  • HLA-DZA
  • HLA-DNA
• Synonyms
  • HLA-D0-alpha
• GenBank acession
  • M31525
• Locus
  6p21.32
• Discovery year
  2001-06-22
• Entrez gene record
  3111
• Pubmed identfication
  • 2370084
• RefSeq identity
  • NM_002119
• Classification
  • C1-set domain containing
  • Histocompatibility complex
• VEGA ID
  OTTHUMG00000031211
• Locus Specific Databases
  • IMGT/HLA Database

MeSH Data

• Name
  Ligase Chain Reaction
• Concept
  Scope note: A DNA amplification technique based upon the ligation of OLIGONUCLEOTIDE PROBES. The probes are designed to exactly match two adjacent sequences of a specific target DNA. The chain reaction is repeated in three steps in the presence of excess probe: (1) heat denaturation of double-stranded DNA, (2) annealing of probes to target DNA, and (3) joining of the probes by thermostable DNA ligase. After the reaction is repeated for 20-30 cycles the production of ligated probe is measured.
• Tree numbers
  • E05.393.620.311
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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