Cathepsin F, human recombinant

• Catalog number
  9232-10
• Price
  Please ask
• Size
  10 µg

• Synonyms
  CATSF, CLN13, CTSF
• Alternative_names
  CATSF, CLN13, CTSF
• Description
  Protease involved in intracellular degradation and turnover of proteins.
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  ≥90%
• Assay
  SDS-PAGE
• Purity
  ≥90%
• Molecular Weight
  This protein is fused with a 6× His tag at N-terminus and has a calculated MW of 26 kDa.
• Storage Temp
  -20°C
• Shipping
  Gel pack
• Shelf Life
  12 months
• Concentration
  1 mg/ml
• Appearance
  Liquid
• Physical form description
  Liquid
• Reconstitution Instructions
  In 20mM Tris-HCl buffer (pH 8.0) containing 0.4M Urea,
• Background Information
  Cathepsin F, also known as CTSF, belongs to the cathepsin family. Cathepsins are papain family cysteine proteinases that represent a major component of the lysosomal proteolytic system. The CTSF gene is ubiquitously expressed, and it maps to chromosome 11q13, close to the gene encoding cathepsin W. CTSF is thought to play a role in normal protein catabolism, and because it is highly expressed in some cancer cell lines, it may be involved in degradative processes occurring during tumor progression. Recombinant human CTSF protein, fused to His-tag at N-terminus, was expressed in E.coli.
• Amino acid sequence
  (237 aa)MGSSHHHHHH SSGLVPRGSH MGSAPPEWDW RSKGAVTKVK DQGMCGSCWA FSVTGNVEGQ WFLNQGTLLS LSEQELLDCD KMDKACMGGLPSNAYSAIKN LGGLETEDDY SYQGHMQSCN FSAEKAKVYI NDSVELSQNE QKLAAWLAKR GPISVAINAF GMQFYRHGIS RPLRPLCSPWLIDHAVLLVG YGNRSDVPFW AIKNSWGTDW GEKGYYYLHR GSGACGVNTM ASSAVVD
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Gene
  CTS protease activity also measured by zymograph electrophoresis of Cathepsins. This cathepsin is supplied in 1.
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Cathepsin  
• Gene symbol
  CTSF
• Short name
  Cathepsin F, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  Cathepsin F, H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:2531
• Gene
  CTSF
• Long gene name
  cathepsin F
• Synonyms
  • CATSF
  • CLN13
• GenBank acession
  • AF071749
• Locus
  11q13.2
• Discovery year
  1998-12-17
• Entrez gene record
  8722
• Pubmed identfication
  • 9822672
  • 10318784
• RefSeq identity
  • NM_003793
• Classification
  • Cathepsins
• VEGA ID
  OTTHUMG00000167090

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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