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Long name
Lefty Primary Polyclonal Antibody
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Also known as
Lefty PAb
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Category
Primary Antibodies
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Conjugation
Unconjugated
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Target Antigen
Lefty
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Specificity
This is a highly specific antibody against Lefty.
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Modification s
None
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Modification site s
Unmodified antibody
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Clonality
Polyclonal antibody
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Clone number
Polyclonal antibody
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Concentration
1ug per 1ul
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Source
This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human Lefty
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Gene ID number
10637
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Tested Applications
WB, IHC-P, IF(IHC-P)
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Recommended dilutions
WB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)
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Cross reactivity
Human, Mouse, Rat
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Cross reactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
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Background of the target antigen
During vertebrate embryogenesis, a left right axis is established. Secreted growth factors of the TGF beta family, including gene products derived from nodal, lefty 1 and lefty 2, play crucial roles in establishing left right asymmetries. TGF beta (Transforming growth factor beta) is a pleiotropic cytokine that regulates growth and differentiation of diverse types of cells. TGF beta actions are directed by ligand induced activation of TGF beta receptors. Complexes formed move into the nucleus, where they act as components of a transcriptional complex. Lefty, a novel member of the TGF beta superfamily, inhibits TGF beta signaling. Lefty acts to inhibit phosphorylation of Smad2 following activation of the TGF beta receptor. Lefty also inhibits events downstream from R Smad phosphorylation. Lefty provides a repressed state of TGF beta responsive genes. The Lefty family is comprised of Lefty 1 and Lefty 2 in mouse, and Lefty A and Lefty B in humans. Members of the TGF beta superfamily require processing for their activation. Cleavage is therefore an essential step for Lefty activation. Lefty is synthesized as a large inactive precursor (41 Kda) that must be endoproteolytically processed to release the bioactive polypeptide (28 kDa and 34 kDa forms). The 28kDa form induces MAPK activity.
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Purification method
This antibody was purified via Protein A.
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Storage conditions
Keep the antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for up to 1 year.
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Synonym names
Protein lefty-1; AI450052; Ebaf; Left right determination factor 1; left right determination factor; Left right determination factor B; left-right determination factor; LEFT-RIGHT DETERMINATION FACTOR 1; LEFTY1; left-right determination, factor B; LEFT-RIGHT DETERMINATION, FACTOR B; LEFTY B; LEFTB; LEFTY 1; Lefty; lefty-1; LEFTY1; LEFTY1, MOUSE, HOMOLOG OF antibody; LEFTYB; Protein lefty-B; RGD1561867; Stimulated by retinoic acid gene 3 protein; STRA3; TGF beta 4; TGF-beta-4; Tgfb4; Transforming growth factor beta 4; Transforming growth factor beta-4.
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Properties
If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
