KLF4 mAntibody (4G6E11)
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Catalog number5300-100
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PricePlease ask
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Size100 µg
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Antibody TargetKLF4
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Target Alternative NameKruppel-like factor 4, GKLF, EZF
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Antibody TypeMonoclonal
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Clone4G6E11
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Species ReactivitiesHuman, Mouse, Rat
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Immunogen SequenceKLF4 antibody was raised against a 20 amino acid peptide near the carboxy terminus of human KLF4.
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AccessionO43474
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Gene Id9314
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AppearanceColorless liquid
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FormLiquid
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Concentration1 mg/ml
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Formulation100 µg (1 mg/ml) in 1X PBS containing 0.02 % sodium azide.
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PurificationAffinity chromatography purified via peptide column
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ApplicationWestern blot, ELISA
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Positive ControlWestern Blot: Mouse liver tissue lysate
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Application And UsagesWestern Blot: 1 - 2 μg/ml, ELISA. However, the optimal conditions should be determined individually.
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DescriptionKLF4 is a transcription factor that functions as both a transcriptional activator and repressor to regulate proliferation and differentiation of multiple cell types. The role of KLF4 in embryonic development sμggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing KLF4 and the transcription factors POU5F1, Sox2, and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing KLF4, Sox2, POU5F1 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine. KLF4 interacts directly with POU5F1 and Sox2 in iPS and ES cells and activates the target gene NANOG.
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Storage Condition-20 °C
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Shipping Conditiongel pack
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Shelf Life12 months
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UsageFor Research Use Only! Not to be used in humans.
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HandlingThe antibody solution should be gently mixed before use.
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Western Blot VerifiedVerified
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Immunocytochemistry VerifiedNot Verified
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Immunofluorescence VerifiedNot Verified
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Immunoprecipitation VerifiedNot Verified
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FACS VerifiedNot Verified
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ELISA VerifiedVerified
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ChIP VerifiedNot Verified
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Flow Cytometry VerifiedNot Verified
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Gene target
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Gene symbolKLF4
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Short nameKLF4 mAntibody (4G6E11)
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IsotypeMouse IgG1
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Alternative nameKLF4 mAntibody (4G6E11)
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Gene info
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Identity
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Gene
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Long gene nameKruppel like factor 4
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Synonyms gene name
- Kruppel-like factor 4 (gut)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1999-12-14
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Zinc fingers C2H2-type
- Kruppel like factors
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data