Human Embryonic Stem Cell HSF-1.14 Cell Line Primary cDNA Library

Description

this 1 cDNA is double stranded complementary DNA derived by AS ONE INTERNATIONAL from mRNA with very high fidelity reverse transcription using a pfu or pfx mix, For cells, cell lines and tissues in culture till half confluency.
Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Test

Stem cell factors and stem cell growth factors will produce stem cells or be part of a transdifferentiation process to produce other cells. A cell can transdifferentiate by going back to the naive stem cell stadium or directly into the other cell, helped by the stem cell and transdifferentiationf actors. Stem cell growth factors or stem cell factors are mostly used to produce iPSCs or induced pluripotent stem cells by Jamaka or Thomson factors by using for example 5 Lenti-III-CMV viruses, expressing the Yamanaka iPSC factor set (Oct4, Sox2, Nanog and Lin28) + GFP positive control. Trans differentiation will omit the stem cell stadium but stem cell factors sill play an important role in trans differentiation strategies.

Gene target

Embryonic Stem HSF-1 Line Primary Library
Gene symbol

HSF1, NR5A1, IL6, IGHVII-1-1, MIR1-1, TRUND-NNN8-1, TRUND-NNN7-1, CDK5RAP3, IGKV1OR2-1, MIR101-1
Short name

Embryonic Stem HSF-1 14 Line Primary cDNA Library
Technique

cDNA, cDNAs
Species

Human, Humans
Alternative name

H. sapiens Embryonic progenitor cellular HSF-1.14 cellular Line captor complementary Desoxyribonucleic acid collection
Alternative technique

pcr
Tissue

cdna, cell, stem

Identity

HGNC:6018
Gene

IL6
Long gene name

interleukin 6
Synonyms gene
- IFNB2
Synonyms gene name
- interleukin 6 (interferon, beta 2)
Synonyms
- IL-6
- BSF2
- HGF
- HSF
Synonyms name
- interferon, beta 2
GenBank acession
- M18403
Locus

7p15.3
Discovery year

1986-01-01
Entrez gene record

3569
Pubmed identfication
- 3294161
RefSeq identity
- NM_000600
Classification
- Interleukins
- Interferons
- Interleukin 6 type cytokine family
VEGA ID

OTTHUMG00000023178

Identity

HGNC:5670
Gene

IGHVII-1-1
Long gene name

immunoglobulin heavy variable (II)-1-1 (pseudogene)
Synonyms gene name
- immunoglobulin heavy variable (II)-1-1
- immunoglobulin heavy variable (II)-1-1 pseudogene
Synonyms
- IGHV(II)-1-1
GenBank acession
- AB019441
Locus

14q32.33
Discovery year

2000-04-17
Entrez gene record

28377
RefSeq identity
- NG_001019
Classification
- Immunoglobulin heavy locus at 14q32.33
VEGA ID

OTTHUMG00000152325

Identity

HGNC:18673
Gene

CDK5RAP3
Long gene name

CDK5 regulatory subunit associated protein 3
Synonyms
- MST016
- FLJ13660
- C53
- IC53
- HSF-27
- OK/SW-cl.114
- LZAP
Synonyms name
- ischemic heart CDK5 activator-binding protein C53
- LXXLL/leucine-zipper-containing ARFbinding protein
GenBank acession
- AF110322
Locus

17q21.32
Discovery year

2002-07-22
Entrez gene record

80279
Pubmed identfication
- 10721722
RefSeq identity
- NM_176096
VEGA ID

OTTHUMG00000178682

Identity

HGNC:5760
Gene

IGKV1OR2-1
Long gene name

immunoglobulin kappa variable 1/OR2-1 (pseudogene)
Synonyms gene
- IGKVPZ1
- IGKV1OR-1
Synonyms gene name
- immunoglobulin kappa variable 1/OR-1
- immunoglobulin kappa variable 1/OR-1 pseudogene
- immunoglobulin kappa variable 1/OR-1 (pseudogene)
Synonyms
- IGKV1/OR-1
- IGKV1/OR2-1
GenBank acession
- Z12367
Locus

2p11.1
Discovery year

2000-04-18
Entrez gene record

3531
Pubmed identfication
- 2853098
Classification
- Immunoglobulin kappa (IGK) orphons
VEGA ID

OTTHUMG00000155081

Name

Embryo Research
Concept

Scope note: Experimentation on, or using the organs or tissues from, a human or other mammalian conceptus during the prenatal stage of development that is characterized by rapid morphological changes and the differentiation of basic structures. In humans, this includes the period from the time of fertilization to the end of the eighth week after fertilization.
Tree numbers
- E05.313
Qualifiers

economics, ethics, history, legislation & jurisprudence