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Category
Primary Antibodies
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Long description
Recognizes a 120kDa protein, identified as the alpha subunit of hypoxia-inducible factor-1 (HIF-1). HIF-1 is a heterodimer consisting of an alpha and a beta subunit, both belonging to the basic-helix-loop-helix Per-aryl hydrocarbon receptor nuclear translocator-Sim (PAS) family of transcription factors. HIF-1 is a key component of a widely operative transcriptional response activated by hypoxia, cobaltous ions, and iron chelation. HIF-1 activates transcription of hypoxia-inducible genes, including those encoding: erythropoietin, vascular endothelial growth (VEGF), heme oxygenase-1, inducible nitric oxide synthase, and the glycolytic enzymes aldolase A, enolase 1, lactate dehydrogenase A, phosphofructokinase I, and phosphoglycerate kinase 1. The C-terminal of HIF-1 alpha binds to p300. p300/CBP-HIF complexes participate in the induction of hypoxia-responsive genes, including VEGF.
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Antibody come from
Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with stimulated HUVEC cells and mouse myeloma p3-NS-1/Ag4-1 cells.
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Other description
Provided as solution in phosphate buffered saline with 0.08% sodium azide. Protein A/G Chromatography
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Clone
H1alpha67
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Antigen antibody binding interaction
Mouse anti Hif-1a Antibody
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Antibody is raised in
Mouse
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Antibody s reacts with
Human, Sheep, Mouse, Rat, Ferret
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
Immunoprecipitation, Western Blot, Immunohistochemistry (Frozen & Paraffin Sections)
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Antibody s suited for
This antibody can be used for flow cytometry (0.5 µg unconjugated Ab/106 cells), immunohistochemistry on frozen tissues, immunoprecipitation (2 µg/mg of protein lysate) and Western blotting (1 µg/ml). Optimal concentration should be evaluated by serial dilutions.
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Storage
-20ºC
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Relevant references
1. Adams DH; et al. Endothelial activation and circulating vascular adhesion molecules in alcoholic liver disease. Hepatology, 1994, 19(3):588-94._x000B__x000B_2. Andersen CB; et al. Acute kidney graft rejection. A morphological and immunohistological study on zero-hour and follow-up biopsies with special emphasis on cellular infiltrates and adhesion molecules. Apmis, 1994, 102(1):23-37._x000B__x000B_3. Belmont HM; et al. Up-regulation of endothelial cell adhesion molecules characterizes disease activity in systemic lupus erythematosus. The Shwartzman phenomenon revisited. Arthritis and Rheumatism, 1994, 37(3):376-83._x000B__x000B_4. Burrows TD; et al. Expression of adhesion molecules by endovascular trophoblast and decidual endothelial cells: implications for vascular invasion during implantation. Placenta, 1994, 15:21-33._x000B__x000B_5. Coleman N; Stanley MA. Characterization and functional analysis of the expression of vascular adhesion molecules in human papillomavirus-related disease of the cervix. Cancer, 1994, 74(3):884-92._x000B__x000B_6. Cunningham AC; et al. Constitutive expression of MHC and adhesion molecules by alveolar epithelial cells (type II pneumocytes) isolated from human lung and comparison with immunocytochemical findings. Journal of Cell Science, 1994, 107:443-9.
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
