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Bovine Angiotensin ELISA Kit 96 well plate

Bovine Angiotensin ELISA Kit 96 well plate is available 9 times from Bioma labs

EKU02393 | Bovine Angiotensin I Converting Enzyme (ACE) ELISA Kit, 96 well plate size: 10x96T | 8,302.68 USD

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EKU02393 | Bovine Angiotensin I Converting Enzyme (ACE) ELISA Kit, 96 well plate size: 96T | 1,089.76 USD

Catalog number EKU02393
Supplier bioma
Price 1,089.76 USD
Size 96T
Verified reactivity Bos taurus; Bovine (Cattle)
Detection limits 6.25-400 ng/mL
Sensitivity limit 2,15 ng/mL
Samples to be analyzed Serum, plasma and other biological fluids.
Assay duration 3 hours
Assay class Sandwich
Alternate protein number Please refer to SwissProt
Gene name Angiotensin I Converting Enzyme
Alternate gene name CD143; ACE1; DCP1; ACEI; ACE-I; Kininase II; Angiotensin-Converting Enzyme; Peptidyl-Dipeptidase A; Dipeptidyl Carboxypeptidase 1; Angiotensin-converting enzyme, soluble form
Estimated production time 7-11 business days
Use for 8 months
ELISA's specificity This assay has high sensitivity and excellent specificity for detection of Angiotensin I Converting Enzyme (ACE). No significant cross-reactivity or interference between Angiotensin I Converting Enzyme (ACE) and analogues was observed.
Assay principle The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Angiotensin I Converting Enzyme (ACE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Angiotensin I Converting Enzyme (ACE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Angiotensin I Converting Enzyme (ACE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Angiotensin I Converting Enzyme (ACE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Precision of the test Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Angiotensin I Converting Enzyme (ACE) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Angiotensin I Converting Enzyme (ACE) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV
ELISA's stability The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Research main area Enzyme & Kinase;Cardiovascular biology;
Properties E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Description Enzymes are cleaving the substrate. If the substrate is DNA they are called restriction enzymes. Activating enzymes will cut off the domain that is biological active to become functional.A microtiter plate (spelled Microtiter is a registered trade name in the United States) or microplate or micro well plate or multiwell, is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories. A very common usage is in the enzyme-linked immunosorbent assay (ELISA), the basis of most modern medical diagnostic testing in humans and animals. A microplate typically has 6, 24, 96, 384 or 1536 sample wells arranged in a 23 rectangular matrix. Some microplates have even been manufactured with 3456 or 9600 wells, and an "array tape" product has been developed that provides a continuous strip of microplates embossed on a flexible plastic tape.
Gene targetAngiotensin Converting ACE
Short name Angiotensin I Converting Enzyme (ACE) ELISA Kit, 96
Technique ELISA kit, Enzyme, ELISA
Alternative name Bovine Angiotensin I Converting Enzyme (Angiotensin-converting enzyme; Uncharacterized protein) Enzyme-linked immunosorbent assay reagent, 96 well plate
Alternative technique enzymes
Alternative to gene target v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832, Angiotensin-converting enzyme; Uncharacterized protein, ACE and IDBG-728302 and ENSG00000264813 and, peptidyl-dipeptidase activity, Plasma membranes
1. Gene info
Identity 2707
Gene ACE
Long gene name angiotensin I converting enzyme
Synonyms gene
  • DCP1
Synonyms gene name
  • angiotensin I converting enzyme (peptidyl-dipeptidase A) 1
Synonyms
  • ACE1
  • CD143
Synonyms name
  • peptidyl-dipeptidase A
GenBank acession
  • J04144
Locus 17q23.3
Discovery year 1989-06-06
Entrez gene record 1636
Pubmed identfication
  • 2554286
  • 10319862
RefSeq identity
  • NM_000789
Classification
  • CD molecules
Havana BLAST/BLAT OTTHUMG00000154927
Product images
    #Bovine#.png

EKU02393 | Bovine Angiotensin I Converting Enzyme (ACE) ELISA Kit, 96 well plate size: 5x96T | 4,594.17 USD

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EKU02402 | Bovine Angiotensin II (AngII) ELISA Kit, 96 well plate size: 96T | 1,089.76 USD

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EKU02402 | Bovine Angiotensin II (AngII) ELISA Kit, 96 well plate size: 10x96T | 8,302.68 USD

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EKU02402 | Bovine Angiotensin II (AngII) ELISA Kit, 96 well plate size: 5x96T | 4,594.17 USD

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EKB01717 | Bovine Angiotensin II competitive ELISA kit, 96 well plate size: 96T | 707.80 USD

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EKB01717 | Bovine Angiotensin II competitive ELISA kit, 96 well plate size: 10x96T | 6,019.32 USD

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EKB01717 | Bovine Angiotensin II competitive ELISA kit, 96 well plate size: 5x96T | 3,135.59 USD

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Verified applications ELISA
ELISA detection Colorimetric
Protein number Please refer to Uniprot
Gene number Please refer to GenBank
Shipping requirements Blue ice
Storage recommendation -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
ELISA's cross-reactivity This assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.
Protocol Please see ELISA's datasheet, otherwise contact us
QC The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
Warnings The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
Notes For research use only. Not for diagnostic procedures.
Host antigen assay ELISA kit 1 Kit (96 Wells)
Species Bovine
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Angiotensin I Converting Enzyme (ACE) ELISA Kit, 96 | Techni
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  • Angiotensin
  • Converting
  • ACE
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