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Synonyms
CD47, MER6, IAP, OA3
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Alternative_names
CD47, MER6, IAP, OA3
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Description
Plays a role in cell adhesion modulation of integrins
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Source
HEK 293 cells
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Purity by SDS PAGE
>95% as determined by SDS-PAGE
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Endotoxin Level
Less than 0.1 EU per ug by the LAL method.
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Activity Specifications test method
Immobilized Mouse CD47, Mouse IgG2a Fc Tag at 10 ug/mL (100 uL/well) can bind Biotinylated Human SIRP alpha, Fc Tag with a linear range of 0.16-5 ug/mL
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Binding Capacity
Immobilized Mouse CD47, Mouse IgG2a Fc Tag at 10 ug/mL (100 uL/well) can bind Biotinylated Human SIRP alpha, Fc Tag with a linear range of 0.16-5 ug/mL
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Molecular Weight
This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 40.7 kDa. The protein migrates as 55-85 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
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Storage Temp
-20°C
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Shipping
Gel Pack
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Shelf Life
12 months
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Appearance
Lyophilized powder
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Physical form description
Lyophilized
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Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in sterile deionized water to a concentration of 100 µg/ml. Do not vortex. It is recommended to store at -20°C.
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Background Information
Leukocyte surface antigen CD47 is also known as Antigenic surface determinant protein OA3, Integrin-associated protein (IAP) and Protein MER6. CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain. CD47 is very broadly distributed on normal adult tissues. CD47 has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins and plays an important role in memory formation and synaptic plasticity in the hippocampus by similarity. CD47 is the receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. CD47 Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation.
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Amino acid sequence
AA Gln 19 - Lys 140
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
CD47
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Short name
CellExp™ CD47, Fc Tag, Mouse Recombinant
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Technique
Recombinant, Mouse, FC, mouses, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
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Host
mouse
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Label
Fc Tag
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Species
Humans, Mouses
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Alternative name
H. sapiens CellExp™ CD47 molecule, fragment c detection labelled, Mouse Rec.
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Alternative technique
rec, murine
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Alternative to gene target
CD47 molecule, IAP and MER6 and OA3, CD47 and IDBG-48744 and ENSG00000196776 and 961, thrombospondin receptor activity, Plasma membranes, Cd47 and IDBG-165137 and ENSMUSG00000055447 and 16423, CD47 and IDBG-631689 and ENSBTAG00000003585 and 282661
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