Heat Shock Protein 70, human recombinant
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Catalog number4859-5000
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Price:
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Size5 mg
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SynonymsHuman Heat Shock Protein 70, Heat Shock Protein 70
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Alternative_namesHuman Heat Shock Protein 70, Heat Shock Protein 70
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DescriptionA chaperone protein that assists protein folding
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RecombinantYes
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SourceE. coli
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Purity by SDS PAGE≥98%
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AssaySDS-PAGE
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Purity≥98%
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Molecular Weight70.0 kDa
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Storage Temp-20°C
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ShippingGel pack
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Shelf Life12 months
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AppearanceLyophilized protein
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Physical form descriptionLyophilized from 41 mM Tris, pH 8 containing 81 mM NaCl.
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Reconstitution InstructionsReconstitute in H₂O (solubility > 40 mg/ml), aliquot and store at -80 °C
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Background InformationHSP70 and HSP90 are molecular chaperones expressed constitutively under normal conditions to maintain protein homeostasis and are induced upon environmental stress. Both HSP70 and HSP90 are able to interact with unfolded proteins to prevent irreversible aggregation and catalyze the refolding of their substrates in an ATP- and co-chaperone-dependent manner. HSP70 has a broad range of substrates including newly synthesized and denatured proteins, while HSP90 tends to have a more limited subset of substrates, most of which are signaling molecules. HSP70 and HSP90 often function collaboratively in a multi-chaperone system, which requires a minimal set of co-chaperones: HSP40, Hop, and p23. The co-chaperones either regulate the intrinsic ATPase activity of the chaperones or recruit chaperones to specific substrates or subcellular compartments. When the ubiquitin ligase CHIP associates with the HSP70/HSP90 complex as a cofactor, the unfolded substrates are subjected to degradation by the proteasome. The biological functions of HSP70/HSP90 extend beyond their chaperone activity. They are essential for the maturation and inactivation of nuclear hormones and other signaling molecules. They also play a role in vesicle formation and protein trafficking.
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolHSPA13, HSPA9
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Short nameHeat Shock Protein 70, recombinant
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHuman, Humans
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Alternative nameHeat Shock Protein 70, H. sapiens Rec.
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Alternative techniquerec
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Gene info
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Identity
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Gene
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Long gene nameheat shock protein family A (Hsp70) member 13
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Synonyms gene
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Synonyms gene name
- stress 70 protein chaperone, microsome-associated, 60kD
- stress 70 protein chaperone, microsome-associated, 60kDa
- heat shock protein 70kDa family, member 13
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Locus
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Discovery year1997-03-21
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Entrez gene record
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Pubmed identfication
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Classification
- Heat shock 70kDa proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameheat shock protein family A (Hsp70) member 9
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Synonyms gene
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Synonyms gene name
- heat shock 70kDa protein 9B (mortalin-2)
- heat shock 70kDa protein 9 (mortalin)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1997-09-12
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNA protein coding host genes
- Heat shock 70kDa proteins
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data