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Alternative name1
Anti-Rabbit anti Monkey IgG Fab, conjugated with Horseradish peroxidase
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Alternative name2
Rabbit anti Monkey IgG Fab, conjugated with Horseradish peroxidase
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Alternative name3
Lapin anti Monkey IgG Fab, conjugated with Horseradish peroxidase
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Alternative name4
Rabbit anti Monkey IgG Fab
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Alternative name5
N/A
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Other names
N/A
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Gene name
N/A
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Gene name synonims
N/A
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Other gene names
N/A
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Category
Secondary Antibodies
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Clonality
Polyclonal
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Immunoglobulin isotype
N/A
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Clone
Polyclonal antibody
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Host organism
Rabbit
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Species reactivity
Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since immunoglobulins of different species frequently share antigenic determinants. Cross-reactivity of this immunoconjugate has not been tested in detail, however in doo
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Specificity and cross reactivity
Horseradish peroxidase-conjugated IgG fraction of polyclonal Rabbit antiSerum to Monkey Fab of IgG. The reactivity of the antiserum is directed to the Fab subunits of intact IgG, IgA IgM and other Ig classes of both y:, light chain types, with their Fab or F(ab')2 subunits, and with free light chains of kappa and lambda type In immunoelectrophoresis and double radial immunodiffusion using various antiserum concentrations against pooled monkey serum a characteristic precipitin line IS obtained, which shows a reaction of identity with the precipitin line of the purified Fab.
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Purification method
Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and_x000D_ purification of the IgG fraction by DEAE-chromatography.
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Form Appearance
Horseradish peroxidase-coupled purified hyperimmune rabbit IgG lyophilized from a solution In phosphate buffered saline (PBS, pH 7.2).
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Concentration
N/A
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Storage and shipping
Handling: The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at_x000D_ or below -20°C. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove Insoluble_x000D_ particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed_x000D_ slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile_x000D_ phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions_x000D_ should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon_x000D_ storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate. Packing: Vial with 1 mllyophilized immunoconjugate. Storage & Shelf Life: Lyophilized at +4°C at least 10 years reconstituted at or below -20°C 3-5 years reconstituted at +4°C 7 days
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Tested for
ELISA (EIA), Immunocytochemistry (IHC), Immunohistochemistry (IHC), Dot Blot (DB), Immunoblotting (IB)
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Description
productone is a polyclonal antibody of high purity and binding affinity for the antigen that it is risen against. Properly used, this antibody will ensure excellent and reproducible results with guaranteed success for the applications that it is tested in. Polyclonal antibodies have series of advantages - larger batches can be supplied at a time, they are inexpensive to manufacture and respectively to buy, the time needed for production is considerably shorter. Polyclonal antibodies generally are more stable and retain their reactivity under unfavorable conditions. To obtain more detailed information on productone, please, refer to the full product datasheet.
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Advisory
In order to retain the quality and the affinity of productone unchanged, please, avoid cycles of freezing and thawing. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
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Additional description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. Rhesus Monkeys proteins are often measured by ELISA on serum or plasma since consensus epitopes with the human ELISA are used for producing the antibodies of these ELISA test kits. Often cDNA of monkeys is used as alternative to human cDNA as a model for drug development. Monkeys are generally considered to be intelligent, particularly Old World monkeys.
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Properties
Human Fab Of Normal IgG Chromatographically purified Fab fragment of human IgG. Purified human Fab may be used as a reference antigen, calibrator, coating protein and blocking agent
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
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Conjugate
Peroxidases with EC number 1.11.1.x are catalyzing the reaction of
ROOR' + electron donor (2 e−) + 2H+ → ROH + R'OH
