Recombinant Human Mothers Against Decapentaplegic Homolog 3/SMAD3 Protein(N-6His-Flag)

• Catalog number
  PKSH032763-10µg
• Price
  Please ask
• Size
  10µg

• Synonym
  Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3; SMAD3; MADH3
• Activity
  NA
• Sequence
  Ser2-Ser425
• Fusion tag
  N-6His-Flag
• Accession
  P84022
• Expressed Host
  E.coli
• Shipping
  The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.
• Purity
  ＞95% as determined by reducing SDS-PAGE.
• Endotoxin
  ＜1.0 EU per µg as determined by LAL test.
• Stability and Storage
  Lyophilized protein should be stored at < -20℃, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7℃ for 2-7 days.Aliquots of reconstituted samples are stable at < -20℃ for 3 months
• Mol Mass
  50.3 kDa
• AP Mol Mass
  60 kDa
• Formulation
  Lyophilized from a 0.2 μm filtered solution of 20mM PB,500mM NaCl, pH7.5.
• Background
  Mothers against decapentaplegic homolog 3(SMAD3) is a cytoplasm protein which belongs to the dwarfin/SMAD family. Smad proteins undergo rapid nuclear translocation upon stimulation by transforming growth factor and in so doing transduce the signal into the nucleus. Receptor-regulated SMAD is an intracellular signal transducer and transcriptional modulator activated by TGF-beta and activin type 1 receptor kinases. SMAD3 binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. It also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. SMAD3 has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive.
• Technical data
• Properties
  An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv.  These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Conjugation
  Flag
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Mothers   Against   Decapentaplegic   Homolog   3/SMAD3   Protein   N-6His  
• Gene symbol
  SMAD3
• Short name
  Recombinant Mothers Against Decapentaplegic Homolog 3/SMAD3 Protein(N-6His- )
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Label
  Flag
• Species
  Human, Humans
• Alternative name
  Rec. H. sapiens Mothers Against Decapentaplegic Homolog 3/SMAD family member 3 Protein(N-6His-Flag)
• Alternative technique
  rec
• Alternative to gene target
  SMAD family member 3, HSPC193 and HsT17436 and JV15-2 and LDS1C and LDS3 and MADH3, SMAD3 and IDBG-18366 and ENSG00000166949 and 4088, transforming growth factor beta receptor, nuclei, Smad3 and IDBG-175693 and ENSMUSG00000032402 and 17127, SMAD3 and IDBG-645466 and ENSBTAG00000012599 and 515125

Gene info

• Identity
  HGNC:6769
• Gene
  SMAD3
• Long gene name
  SMAD family member 3
• Synonyms gene
  • MADH3
• Synonyms gene name
  • MAD, mothers against decapentaplegic homolog 3 (Drosophila)
  • SMAD, mothers against DPP homolog 3 (Drosophila)
• Synonyms
  • JV15-2
  • HsT17436
• GenBank acession
  • BC050743
• Locus
  15q22.33
• Discovery year
  1996-11-15
• Entrez gene record
  4088
• Pubmed identfication
  • 8774881
  • 8673135
• RefSeq identity
  • NM_005902
• Classification
  • SMAD family
• VEGA ID
  OTTHUMG00000133230
• Locus Specific Databases
  • The Globin Gene Server

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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