Goat anti Rat IgG (H + L) (Fab'2) (FITC)
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Catalog number
MBS538847
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Price
Please ask
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Size
0.5 mg
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Products_type
Secondary Antibody
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Products_short_name
[Goat anti Rat IgG (H + L) (Fab'2) (FITC)]
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Products_name_syn
[Goat anti Rat Immunoglobulin G (H + L) secondary (Fab'2) (FITC)]
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Products_gene_name
[IgG]
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Reactivity
Rat IgG (H + L)
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Specificity
Rat IgG (H + L)
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Purity
Goat anti Rat IgG (H + L) (Fab'2) (FITC) was purified by affinity chromatography.
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Form
Lyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA, and 0.01% NaN3. Immunoglobulin and protease free.
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Storage_stability
Store at 4 degree C until reconstitution. Following reconstitution aliquot and freeze at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
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Tested_application
ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Properties
Human Fab Of Normal IgG Chromatographically purified Fab fragment of human IgG. Purified human Fab may be used as a reference antigen, calibrator, coating protein and blocking agent, This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Latin name
Capra aegagrus hircus, Rattus norvegicus
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Gene target
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Gene symbol
FUT1, L3MBTL2
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Short name
Goat anti IgG (H + L) (Fab'2) (FITC)
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Technique
anti, FITC, Goat, Fab, IgG, antibody to, fabs, Fluorescein, goats, IgGs
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Host
Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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Isotype
fab'2, IgG, Fab fragment, IgG
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Label
FITC
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Species
Rat, Goats, Rats
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Alternative name
caprine antibody to Rat Immunoglobulin G (H + L) (Fab'2) (fluorecein)
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Alternative technique
antibodies, caprine, fluorescine, seconairy, immunoglobulins
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Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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