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Available ordering format
Lyophilized
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Immunogen
KLH-conjugated synthetic peptide conserved in known higher plant HSC70 proteins including three isoforms of Arabidopsis thaliana HSC70-1 (NP_ 001119156.1), HSC70-2 (NP_195869.1) and HSC70-3 (NP_187555.1) as well as heat shock inducible Hsp70 of Arabidopsis thaliana AT3g12580/T2E22_110 and At1g16030 and AT3g12580/T2E22_110
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Serum
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How to reconstitute
For reconstitution add 50 µl of sterile water.
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB), immunoprecipitation (IP)
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Connected products
AS08 348 | anti-chloroplastic HSP70AS08 347 | anti-mitochondrial HSP70Plant and algal protein extraction bufferSecondary antibodies
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Recommended dilutions for use
1: 3000 with standard ECL on 5 µg of protein per well (WB), 2-3 µl/protein extract of concentration 3-5 mg/ml
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Molecular weight expected аpparent
70 kDa
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Verified reactivity
Arabidopsis thaliana, Cucumis sativus, E. teft, Hordeum vulgare, Medicago sativa, Silene vulgaris, Solanum lycopersicum, Pinus strobus, Polyscias elegans, Zea mays, algae: Desmodesmus subspicatus, phycobiont: Trebouxia TR1 and TR9, Plasmodium falciparum
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Possible reactivity
Ageratina adenophora, Allium sativum, Arabis alpina, Arachis diogoi, Arundo donax, Brassica napus, brassica rapa subsp. pekinensis, Camellia sinensis, Chlamydomonas reinhardti, Citrus sinensis, Coffea arabica, Eriobotrya japonica, Gossypium arboretum, Glycine max, Glycine soja, Hordeum vulgare var. distichum, Lotus japonicus, Medicago sativa, medicago truncatula, Musa acuminata subsp. malaccensis, Nicotiana tabacum, Nicotiana bethamiana, Oryza sativa, Phaseolus vulgaris, Physcomitrella patens, Pinus taeda, Pisum sativum, Populus balsamifera, Populus trichocarpa, Salix gilgiana, Saussurea medusa,Solanum tuberosum, Solanum commersonii, Spinacia oleracea, Tragopogon dubius, Tragopogon porrifolius, Triticum aestivum, Vitis vinifera, Volvox sp.
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No reactivity
no confirmed exceptions from predicted reactivity known in the moment
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Supplementary information
This product can be sold containing ProClin if requested
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References
Shen et al. (2016). The Arabidopsis polyamine transporter LHR1/PUT3 modulates heat responsive gene expression by enhancing mRNA stability. Plant J. 2016 Aug 19. doi: 10.1111/tpj.13310. [Epub ahead of print] Gorovits et al. (2016). Tomato yellow leaf curl virus confronts host degradation by sheltering in small/midsized protein aggregates. Virus Res. 2016 Feb 2;213:304-13. doi: 10.1016/j.virusres.2015.11.020. Epub 2015 Dec 1. Ghandi et al. (2016). Tomato yellow leaf curl virus infection mitigates the heat stress response of plants grown at high temperature. Sci Rep. 2016 Jan 21;6:19715. doi: 10.1038/srep19715. Kim et al. (2015). Cytosolic targeting factor AKR2A captures chloroplast outer membrane-localized client proteins at the ribosome during translation. Nat Commun. 2015 Apr 16;6:6843. doi: 10.1038/ncomms7843. Hattab et al. (2015). Characterisation of lead-induced stress molecular biomarkers in Medicago sativa plants. Environm. Exp. Botany. Volume 123, March 2016, Pages 1-12. Derbyshire et al. (2015). Proteomic Analysis of Microtubule Interacting Proteins over the Course of Xylem Tracheary Element Formation in Arabidopsis. Plant Cell. 2015 Oct 2. pii: tpc.15.00314. Law et al. (2015). Phosphorylation and Dephosphorylation of the Presequence of pMORF3 During Import into Mitochondria from Arabidopsis thaliana. Plant Physiol. 2015 Aug 24. pii: pp.01115.2015. Moshe at al. (2015). Tomato plant cell death induced by inhibition of HSP90 is alleviated by Tomato yellow leaf curl virus infection. Mol Plant Pathol. 2015 May 12. doi: 10.1111/mpp.12275. Piechota et al. (2015). Unraveling the functions of type II-prohibitins in Arabidopsis mitochondria. Plant Mol Biol. 2015 Apr 21. Hazlina et al. (2015). Photoinhibition and Development of Stress Proteins in Macroalgae Irradiated with Ultraviolet Radiation. ASM Sci. J., 7(2), 118-128. Guggisberg et al. (2014). A sugar phosphatase regulates the methylerythritol phosphate (MEP) pathway in malaria parasites. Nat Commun. 2014 Jul 24;5:4467. doi: 10.1038/ncomms5467. Liu et al. (2014). Spermidine Enhances Waterlogging Tolerance via Regulation of Antioxidant Defence, Heat Shock Protein Expression and Plasma Membrane H+-ATPase Activity in Zea mays. J. Agronomy and Crop Science, Article first published online: 1 APR 2014, DOI: 10.1111/jac.12058.
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Scientific context
Heat-shock protein 70 (Hsp70) is the major stress-inducible protein in vertebrates and is highly conserved throughout evolution. It plays a role as a molecular chaperone and is important for allowing cells to cope with acute stressor insult, especially those affecting the protein machinery. Heat shock cognate protein 70 (HSC70), is a highly conserved protein and a member of the family of molecular chaperones.
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Notes
Immunoprecipitation protocol using Agrisera anti-Hsp70 cytosolic antibodies can be found here.
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Protein number
Q9LHA8
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TAIR number
AT3G12580
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