CD73/NT5E Antibody (CT)

• Catalog number
  6802-100
• Price
  Please ask
• Size
  100 μl

• Antibody Target
  CD73/NT5E
• Target Alternative Name
  NT5E; NT5; NTE; 5'-nucleotidase; Ecto-5'-nucleotidase
• Antibody Type
  Polyclonal
• Species Reactivities
  Human, Mouse.
• Immunogen Sequence
  This CD73 (NT5E) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 520-550 amino acids from the C-terminal region of human CD73 (NT5E).
• Accession
  P21589
• Gene Id
  4907
• Appearance
  Colorless liquid
• Form
  Liquid
• Formulation
  In PBS with 0.09% (W/V) sodium azide.
• Purification
  Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
• Application
  Western blot, IHC.
• Positive Control
  Western blot: mouse brain tissue lysate, NT5E 293 ceel lysate. IHC: human placenta tissue.
• Application And Usages
  WB: 1:1000, IHC: 1:10 – 1:50.
• Description
  CD73 (also designated ecto-5'-nucleotidase, E5NT, NT, NT5, NTE, eN and eNT) is a glycosyl-phosphatidylinositol (GPI)-anchored adhesion protein that catalyzes the dephosphorylation of extracellular purine and pyrimidine nucleotides to their corresponding bioactive nucleosides. CD73 is a dimer of two identical subunits that depends on GPI to link with the external face of the plasma membrane. Similar to other GPI-anchored proteins, CD73 mediates co-stimulatory signals in T cell activation. CD73 has few structural variants, yet elicits diverse biological function through differential regulation in endothelial cells (EC), subpopulations of B and T cells, germinal center follicular dendritic cells and on thymic medullary reticular fibroblasts. For example, IgG mediated neutralization of CD73 interferes with lymphocyte adhesion to EC, and blocks aggregation of germinal center B cells and follicular dendritic cells. Furthermore, IgG-mediated targeting of lymphocyte CD73, but not of endothelial cell CD73, causes shedding of CD73 and tyrosine phosphorylation of proteins.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  CD73/NT5E  
• Gene symbol
  NT5E
• Short name
  CD73/NT5E Antibody (CT)
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  CD73/5'-nucleotidase, ecto (CD73) (antibody to-) (CT)
• Alternative technique
  antibodies
• Alternative to gene target
  5'-nucleotidase, ecto (CD73), CALJA and CD73 and E5NT and eN and eNT and NT and NT5 and NTE, NT5E and IDBG-93979 and ENSG00000135318 and 4907, hydrolase activity, Plasma membranes, Nt5e and IDBG-190005 and ENSMUSG00000032420 and 23959

Gene info

• Identity
  HGNC:8021
• Gene
  NT5E
• Long gene name
  5'-nucleotidase ecto
• Synonyms gene
  • NT5
• Synonyms gene name
  • 5' nucleotidase (CD73)
  • 5'-nucleotidase, ecto (CD73)
• Synonyms
  • CD73
  • eN
  • eNT
  • CALJA
• GenBank acession
  • X55740
• Locus
  6q14.3
• Discovery year
  1989-02-23
• Entrez gene record
  4907
• Classification
  • CD molecules
  • 5'-nucleotidases
• VEGA ID
  OTTHUMG00000015139

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

Similar products