Cas9 Monoclonal Antibody
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Catalog numberA1069-50
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Price:
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Size50 µg
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Antibody TargetCas9
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Target Alternative NameCRISPR-associated endonuclease Cas9/Csn1, CRISPR associated protein 9, SpyCas9
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Antibody TypeMonoclonal
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Clone7A9
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Immunogen SequenceN-terminal fragment of Streptococcus pyogenes Cas9.
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AccessionQ99ZW2
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Gene Id901176
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AppearanceColorless liquid
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FormLiquid
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FormulationPBS containing 0.02% sodium azide.
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PurificationAffinity purified using protein G
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ApplicationIF, IP, WB
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Positive ControlIF, IP, WB
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Application And UsagesImmunofluorescence: 1:500-1:2,000; Immunoprecipitation: 1:20; Western Blot: 1:1,000
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DescriptionCas9 (CRISPR associated protein 9) is an RNA-guided DNA endonuclease enzyme associated with the CRISPR (Clustered Regularly Interspersed Palindromic Repeats) adaptive immunity system in Streptococcus pyogenes and other bacteria. Cas9 is used to interrogate and cleave foreign DNA, such as invading bacteriophage DNA or plasmid DNA. This is accomplished by unwinding foreign DNA and checking for if it is complementary to the 20 base pair spacer region of the guide RNA. If the DNA substrate is complementary to the guide RNA, Cas9 cleaves the invading DNA.
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Storage Condition-20°C
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Shipping Conditiongel pack
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Shelf Life12 months
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UsageFor Research Use Only! Not to be used in humans
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HandlingThe antibody solution should be gently mixed before use
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Western Blot VerifiedVerified
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Immunocytochemistry VerifiedNot Verified
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Immunofluorescence VerifiedVerified
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Immunoprecipitation VerifiedVerified
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ELISA VerifiedNot Verified
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ChIP VerifiedNot Verified
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Dot Blot VerifiedNot Verified
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Flow Cytometry VerifiedNot Verified
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PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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French translationanticorps
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Gene target
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Short nameCas9 Monoclonal Antibody
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TechniqueAntibody, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies
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IsotypeMouse IgG1κ
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Alternative nameCas9 monoclonal (antibody to-)
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Alternative techniqueantibodies
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MeSH Data
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Name
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ConceptScope note: Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment.
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Tree numbers
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