H3, Histone H3 (affinity purified)

• Catalog number
  AS10 710A
• Price
  Please ask
• Size
  50 µg

• Available ordering format
  Lyophilized in PBS pH 7.4
• Immunogen
  KLH-conjugated synthetic peptide derived from known H3 sequences, inluding Arabidopsis thaliana H3.3 P59169 (At4g40030,At4g40040,At5g10980), H3.2 P59226(At1g09200,At3g27360,At5g10390,At5g10400,At5g65360), H3-like 2 Q9FXI7 (At1g19890)
• Raised in
  Rabbit
• Clonality
  Polyclonal
• Clone
  Polyclonal
• Purification
  Affinity purified serum
• How to reconstitute
  For reconstitution add 50 µl of sterile water.
• Storage condition
  Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
• Verified applications
  ChIp-qPCR (ChIp-qPCR), western blot (WB)
• Connected products
  AS11 1804 | anti-RNA polymerase II subunit B1, rabbit antibodyAS16 3197 | anti-H3 | Histone 3 core, monoclonal antibodyAS15 2855 | anti-H3 | histone H3, chicken antibodyAS10 710 | anti-H3 | histone H3, rabbit antibody (serum)AS10 710PRE | H3 | histone H3, pre-immune serumCollection of antibodies to epigenetics/DNA methylationPlant and algal protein extraction buffer Secondary antibodies
• Recommended dilutions for use
  1 : 5000 with standard ECL (WB), 2.5 µg/100 µg of chromatin (ChIp-qPCR)
• Molecular weight expected аpparent
  15 | 17 kDa
• Verified reactivity
  Arabidopsis thaliana
• Possible reactivity
  Brassica oleracea, Capsicum annuum, Chlamydomonas acidophila, Chlamydomonas reinhardtii, Physcomitrella patens, Salicornia europaea, Solanum lycopersicum, Solanum sogarandinum, Solanum tuberosum, Vicia faba, Zea mays Brachypodium distachyon, Brassica napus, Hordeum vulgare, Nicotiana tabacum, Malus domestica, Medicago sativa, Triticum aestivum, Pinus pinaster, Pisum sativum, Oryza sativa, Zea mays, Vitis vinifera, Volvox sp.
• No reactivity
  no confirmed exceptions from predicted reactivity known in the moment
• Supplementary information
  Protocol for isolation of cytosolic and nuclear fractions can be found here.Specific protol for ChIP can be found here: Saleh et al. (2008).
• References
  To be added when available, antibody released in April 2016.
• Scientific context
  Histone 3 (H3) located in nuclei, incorporated into chromatin. Present in nucleosome together with H2A, H2B and H4.
• Notes
  cellular [compartment marker] of nucleoplasm, loading control antibody for Chlamydomonas reinhardtii
• Protein number
  P59169 , P59226 , Q9FXI7
• TAIR number
  AT4G40030
• Hyperlink
• Test
  A high affinity purification column was use to purify H3, Histone H3 ( ) by agisera by chromatographic size exclusion.
• Description
  1 of protein A or G purified by agisera will give more specificity than crude serum.

• Gene target
  Histone  
• Gene symbol
  H3-4, H3C15, H3-7
• Short name
  H3, Histone H3 ( )
• Technique
  affinity, purified, purifications
• Alternative name
  H3, Histone H3 (protein enriched)
• Alternative technique
  pure

Gene info

• Identity
  HGNC:4778
• Gene
  H3-4
• Long gene name
  H3.4 histone
• Synonyms gene
  • H3FT
  • HIST3H3
• Synonyms gene name
  • H3 histone family, member T
  • histone 3, H3
  • histone cluster 3, H3
  • histone cluster 3 H3
• Synonyms
  • H3t
  • H3/g
  • H3.4
• GenBank acession
  • Z49861
• Locus
  1q42.13
• Discovery year
  1998-09-30
• Entrez gene record
  8290
• Pubmed identfication
  • 8834248
  • 12408966
• RefSeq identity
  • NM_003493
• Classification
  • H3 histones
• VEGA ID
  OTTHUMG00000040044

Gene info

• Identity
  HGNC:20505
• Gene
  H3C15
• Long gene name
  H3 clustered histone 15
• Synonyms gene
  • HIST2H3A
• Synonyms gene name
  • histone 2, H3a
  • histone cluster 2, H3a
  • histone cluster 2 H3 family member a
• Synonyms
  • H3/n
  • H3/o
• GenBank acession
  • AF531305
• Locus
  1q21.2
• Discovery year
  2003-02-19
• Entrez gene record
  333932
• Pubmed identfication
  • 12408966
  • 15527963
• Classification
  • H3 histones
• VEGA ID
  OTTHUMG00000012094

Gene info

• Identity
  HGNC:32060
• Gene
  H3-7
• Long gene name
  H3.7 histone (putative)
• Synonyms gene
  • HIST2H3PS2
  • H3-2
• Synonyms gene name
  • histone 2, H3, pseudogene 2
  • histone cluster 2, H3, pseudogene 2
  • histone cluster 2 H3 pseudogene 2
  • H3.2 histone (putative)
• Synonyms
  • p06
  • H3.7
• GenBank acession
  • AL109948
• Locus
  1q21.1
• Discovery year
  2006-06-12
• Entrez gene record
  440686
• Pubmed identfication
  • 28374988
• RefSeq identity
  • NM_001355409
• Classification
  • H3 histones
• VEGA ID
  OTTHUMG00000041033

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