Mouse Anti-Mouse Neuroligin 4 Monoclonal IgG1, Clone S98-7
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Catalog numberSMC-469D-A390
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PricePlease ask
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Size100 µg
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CloneS98-7
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ImmunogenFusion protein amino acids 782-945 (intracellular C-terminus) of mouse Neuroligin 4. ~45% identity with Neuroligin-1, -2 and -3.
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Antibody s full descriptionMouse Anti-Mouse Neuroligin 4 Monoclonal IgG1 Antibody, Clone: S98-7: ATTO 390
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameASPGX2 Antibody, AUTSX2 Antibody, HLNX Antibody, HNLX Antibody, KIAA1260 Antibody, MGC22376 Antibody, NLGN Antibody, NLGN4 Antibody, NLGN4X Antibody, neuroligin-4 X-linked Antibody, neuroligin X Antibody, neuroligin 4 Antibody, X-linked Antibody
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Raised inMouse
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Antibody s targetNeuroligin 4
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Primary research fieldsNeuroscience, Cell Structure, Post-Synaptic Markers
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BrandnameNone
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Antibodies applicationsWB, IHC, ICC/IF
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Antibody s reactivityMouse
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Antibody s dilutionsWB (1:1000), IHC (1:100); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH 7.4, 50% glycerol, 0.1% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~125-130kDa. Does not cross-react with other Neuroligins.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysis1 µg/ml of SMC-469 was sufficient for detection of Neuroligin 4 in 20 µg of mouse brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cellCell Membrane , Cell Junction
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextThis gene encodes a member of a family of neuronal cell surface proteins. Members of this family may act as splice site-specific ligands for beta-neurexins and may be involved in the formation and remodeling of central nervous system synapses. The encoded protein interacts with discs, large (Drosophila) homolog 4 (DLG4). Mutations in this gene have been associated with autism and Asperger syndrome. Two transcript variants encoding the same protein have been identified for this gene.
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Released date20-Nov-2013
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberABS19580
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Gene number100113365
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Protein numberB0F2B4
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunohistochemistry analysis using Mouse Anti-Neuroligin 4 Monoclonal Antibody, Clone S98-7 (SMC-469). Tissue: Dentate Gyrus. Species: Mouse. Fixation: formaldehyde-fixed paraffin embedded. Primary Antibody: Mouse Anti-Neuroligin 4 Monoclonal Antibody (SMC-469) at 1:100. Secondary Antibody: FITC Goat Anti-Mouse (green). Counterstain: DAPI (blue) nuclear stain at 1:1000. Courtesy of: Rachel Reith, NIH/NIMH. Western Blot analysis of Rat Brain Membrane showing detection of ~125-130 kDa Neuroligin 4 protein using Mouse Anti-Neuroligin 4 Monoclonal Antibody, Clone S98-7 (SMC-469). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain Membrane. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Neuroligin 4 Monoclonal Antibody (SMC-469) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~125-130 kDa. Mouse Anti-Neuroligin 4 Antibody [S98-7] used in Immunohistochemistry (IHC) on Mouse Dentate Gyrus (SMC-469) Mouse Anti-Neuroligin 4 Antibody [S98-7] used in Western Blot (WB) on Rat Brain Membrane (SMC-469)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Gene target
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Gene symbolPIRC34, PIRC33, PIRC32, SNORD114-7, IGHV3OR15-7, SNORD115-7, RNU6-7, PIRC29, PIRC30, PIRC31
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Short nameMouse Anti-Mouse Neuroligin 4 Monoclonal IgG1, Clone S98-7
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Techniqueanti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG1, IgG1
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LabelATTO 390
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SpeciesMouse, Mouses
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Alternative nameMouse Antibody toMouse Neuroligin 4 monoclonal IgG1, clonality S98-7
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Alternative techniquemurine, antibodies
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Clone nameS98-7
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Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 34
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 33
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 32
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 114-7
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Synonyms
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Locus
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Discovery year2006-09-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 3/OR15-7 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable 3/OR15-7
- immunoglobulin heavy variable 3/OR15-7 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-17
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Entrez gene record
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Classification
- Immunoglobulin heavy (IGH) orphons
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 115-7
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene nameRNA, U6 small nuclear 7
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Synonyms
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Locus
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Discovery year2008-06-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nuclear RNAs
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 29
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 30
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 31
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
