Rabbit Anti-Sheep IgG(H+L) IGHG1 Polyclonal Antibody-FITC
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Catalog number
OASB02107
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Price
Please ask
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Size
2X1 mg
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Recognized antigen
IGHG1
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Product type
Antibody
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Gene name
IGHG1
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Long name
Antibody against IGHG1
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Storage
For shorter time periods the IGHG1 Antibodies may be kept refrigerated at +4 degrees Celsius. For extended periods of time keep frozen at -20 °C. Avoid freezing and thawing the lysate repeatedly as these cycles will cause denaturation and/or disruption of the protein chains and peptide bonds of the IGHG1 Antibodies which might cause change or loss of the desired properties.
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Tips
Small amounts of the IGHG1 antibody may get entrapped on the walls or lid of the vial as a result of shaking during transportation or handling. Prior to use, cetrifuge the vial to ensure that all of its content is collected on the bottom. In order to avoid cycles of freezing and thawing we recommend to plan and prepare aliquots according to the experiment demand and defrost only the amount which wil be needed for immediate use.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by aviva they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This aviva Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. aviva FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by aviva. Rabbit antibodies are very stable and can be stored for several days at room temperature. aviva adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Group
Polyclonals and antibodies
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Latin name
Oryctolagus cuniculus
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French translation
anticorps
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Gene target
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Gene symbol
IGHG1
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Short name
Anti-IGHG1
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Technique
Polyclonal, Antibody, Rabbit, anti-, anti, FITC, IgG, antibody to, antibodies, antibodies against human proteins, antibodies for, Fluorescein, IgGs, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit, Rabbits
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Isotype
IgG, IgG
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Label
FITC
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Species
Sheep, The sheep (Ovis aries) ELISAs are detecting sheep proteins and antigens. Some epitopes will also recognize goat proteins. Sheep and goats are also used to produce polyclonals. An adult female sheep is referred to as a ewe a male as a ram, a castrated male as a wether, and a younger sheep as a lamb. Sheeps
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Alternative name
IGHG1 Antibodies
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Alternative technique
polyclonals, antibodies, rabbit-anti, fluorescine, immunoglobulins
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Alternative to gene target
immunoglobulin heavy constant gamma 1 (G1m marker), IGHG1 and IDBG-124377 and ENSG00000211896 and 102723479,102725426,102725526, protein binding, Extracellular
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Gene info
MeSH Data
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Name
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Concept
Scope note:
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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