GOT2, human recombinant

• Catalog number
  7809-100
• Price
  Please ask
• Size
  100 ug

• Synonyms
  Aspartate aminotransferase 2, mitochondrial, KAT4, KATIV, mitAAT
• Alternative_names
  Aspartate aminotransferase 2, mitochondrial, KAT4, KATIV, mitAAT
• Description
  Plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles.
• Recombinant
  Yes
• Source
  E. coli.
• Purity by SDS PAGE
  ≥95%
• Assay
  SDS-PAGE
• Biological activity
  Specific activity is > 44 unit/mg, in which one unit will convert 1.0 µmole of alpha-ketoglutarate to L-Glutamate per minute at pH 8.0 at 25ºC.
• Results
  Specific activity is > 44 unit/mg
• Unit Definition
  One unit will convert 1.0 µmole of alpha-ketoglutarate to L-Glutamate per minute at pH 8.0 at 25ºC.
• Molecular Weight
  48.4 kDa (433 aa, 1-413 aa + His Tag)
• Storage Temp
  -20°C
• Shipping
  gel pack
• Shelf Life
  12 months
• Concentration
  0.5 mg/ml
• Appearance
  Liquid
• Physical form description
  0.5 mg/ml solution 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, 150 mM NaCl and 1 mM DTT.
• Background Information
  GOT2, also known as glutamate oxaloacetate transaminase 2, belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Glutamate oxaloacetate transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Recombinant human GOT2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Amino acid sequence
  MGSSHHHHHH SSGLVPRGSH MGSSSWWTHV EMGPPDPILG VTEAFKRDTN SKKMNLGVGA YRDDNGKPYV LPSVRKAEAQ IAAKNLDKEY LPIGGLAEFC KASAELALGE NSEVLKSGRF VTVQTISGTG ALRIGASFLQ RFFKFSRDVF LPKPTWGNHT PIFRDAGMQL QGYRYYDPKT CGFDFTGAVE DISKIPEQSV LLLHACAHNP TGVDPRPEQW KEIATVVKKR NLFAFFDMAY QGFASGDGDK DAWAVRHFIE QGINVCLCQS YAKNMGLYGE RVGAFTMVCK DADEAKRVES QLKILIRPMY SNPPLNGARI AAAILNTPDL RKQWLQEVKV MADRIIGMRT QLVSNLKKEG STHNWQHITD QIGMFCFTGL KPEQVERLIK EFSIYMTKDG RISVAGVTSS NVGYLAHAIH QVTK
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  GOT2  
• Gene symbol
  GOT2
• Short name
  GOT2, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  GOT2, H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:4433
• Gene
  GOT2
• Long gene name
  glutamic-oxaloacetic transaminase 2
• Synonyms gene name
  • glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2)
  • glutamic-oxaloacetic transaminase 2, mitochondrial
• Synonyms
  • mitAAT
  • KATIV
  • KAT4
  • KYAT4
• Synonyms name
  • kynurenine aminotransferase IV
  • aspartate aminotransferase 2
  • aspartate transaminase 2
• Locus
  16q21
• Discovery year
  2001-06-22
• Entrez gene record
  2806
• Pubmed identfication
  • 17442055
• RefSeq identity
  • NM_002080
• VEGA ID
  OTTHUMG00000133769

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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