UHRF1 polyclonal antibody (ChIP Grade)
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Catalog number
BS7227-50ul
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Price
Please ask
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Size
50ul
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Alternative Name
E3 ubiquitin-protein ligase UHRF1; Nuclear protein 95; Nuclear zinc finger protein Np95; HuNp95; hNp95; RING finger protein 106; Transcription factor ICBP90; hUHRF1; ICBP90; NP95; RNF106
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Immunogen
Recombinant full length Human UHRF1.
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Swiss Prot
Q96T88
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Reactivity
Human
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Applications
WB IHC IF
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Application_all
WB: 1:500~1:2000 IHC/IF: 1:50~1:200
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Product
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
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Purification Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
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Storage Stability
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Specificity
UHRF1 polyclonal antibody detects endogenous levels of UHRF1 protein.
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Usage
Western blot analysis of UHRF1 polyclonal antibody
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BiowMW
~ 90, 110 kDa
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Note
For research use only, not for use in diagnostic procedure.
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Properties
If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Precipitation of Chromatins is a pull down technique of native DNA sequences by specific binding proteins on which on the other side of the protein the antibody can bind. Lots of antibodies are not suited for Chip analysis pulldown because they bind on the binding site of the DNA. The Bioworld antibody is proven to not bind on the DNA binding site and is suitable for ChiP precipitation supplied in 1 vials.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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French translation
anticorps
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Gene target
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Gene symbol
UHRF1
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Short name
UHRF1 polyclonal antibody (ChIP Grade)
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Technique
Polyclonal, Antibody, ChiP, antibodies against human proteins, antibodies for, Chromatine immuno Precipitationg antibody, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit
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Alternative name
UHRF1 polyclonal (antibody to-) (ChIP Grade)
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Alternative technique
polyclonals, antibodies, epigenetics
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Gene info
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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