Catalase, Human Recombinant

• Catalog number
  P1159-100
• Price
  Please ask
• Size
  100 ug

• Synonyms
  CAT
• Alternative_names
  CAT
• Description
  A key antioxidant enzyme in the bodies’ defense against oxidative stress
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  ≥90%
• Assay
  SDS-PAGE
• Activity Specifications test method
  Measured by its ability to decomposes 1.0 µmole of H2O2 per minute at pH 7.0 at 25°C.
• Biological activity
  Specific activity is >20,000 unit/mg
• Results
  >20,000 unit/mg
• Molecular Weight
  62.5 kDa (547 aa, 1-552 aa + His Tag).
• Storage Temp
  -20°C
• Shipping
  Gel Pack
• Shelf Life
  12 months
• Appearance
  Lyophilized
• Physical form description
  Propriety buffer
• Reconstitution Instructions
  Centrifuge the vial prior to opening. Reconstitute in deionized water, store at 4°C and use within 2 weeks after reconstitution.
• Background Information
  CAT is a key antioxidant enzyme in the bodies’ defense against oxidative stress. Also, it is a heme enzyme that is present in the peroxisome of nearly all aerobic cells. CAT converts the reactive oxygen species hydrogen peroxide to water and oxygen and thereby mitigates the toxic effects of hydrogen peroxide. Recombinant human CAT protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Amino acid sequence
  MGSSHHHHHHSSGLVPRGSHMDDDDKADSRDPASDQMQHWKEQRAAQKADVLTTGAGNPVGDKLNVITVGPRGPLLVQDVVFTDEMAHFDRERIPERVVHAKGAGAFGYFEVTHDITKYSKAKVFEHIGKKTPIAVRFSTVAGESGSADTVRDPRGFAVKFYTEDGNWDLVGNNTPIFFIRDPILFPSFIHSQKRNPQTHLKDPDMVWDFWSLRPESLHQVSFLFSDRGIPDGHRHMNGYGSHTFKLVNANGEAVYCKFHYKTDQGIKNLSVEDAARLSQEDPDYGIRDLFNAIATGKYPSWTFYIQVMTFNQAETFPFNPFDLTKVWPHKDYPLIPVGKLVLNRNPVNYFAEVEQIAFDPSNMPPGIEASPDKMLQGRLFAYPDTHRHRLGPNYLHIPVNCPYRARVANYQRDGPMCMQDNQGGAPNYYPNSFGAPEQQPSALEHSIQYSGEVRRFNTANDDNVTQVRAFYVNVLNEEQRKRLCENIAGHLKDAQIFIQKKAVKNFTEVHPDYGSHIQALLDKYNAEKPKNAIHTFVQSGSHLAAREKANL
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Catalase  
• Gene symbol
  CAT
• Short name
  Catalase, Recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  Catalase, H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:1516
• Gene
  CAT
• Long gene name
  catalase
• GenBank acession
  • AY028632
• Locus
  11p13
• Discovery year
  2001-06-22
• Entrez gene record
  847
• RefSeq identity
  • NM_001752
• VEGA ID
  OTTHUMG00000044353

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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