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ELISA category
Streptavidin coating kit Kit
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ELISA detection limits
N/A
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ELISA sensitivity
N/A
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ELISA s description
Streptavidin binds very tightly to the small molecule Biotin. Streptavidin coating of solid phases offers a universal immobilization principle for the detection and analysis of proteins, peptides, PCR-fragments, haptens etc., which must be present in biotinylat-ed form. As a further development BioTeZ offers a polymerized Streptavidin, the Polystreptavi-din R, characterized by an extraordinary high Biotin binding capacity. Coatings made of Polystrept R combine the excellent binding capacity with a high chemo and thermo stability. It is very suitable for coating membranes, beads, biochips, plastics etc. Polystreptavidin R is part of this set of reagents, which has been supplemented by a special solution for pre-coating. The additional pre-coating serves to improve the out-come and should be used especially for very inert surfaces to ensure a high quality coating with Polystreptavidin R. The coating procedure starts with the pre-coating step. The kit is used for adsorptive coating microplates, beads, membranes etc. made of metal or plastics like Polystyrene, Polyethylene, Polypropylene, Polycarbonate, Poly(methyl methacry-late) PMMA or Polyamides with Polystreptavidin “Maximum Capacity”.
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Research area interests
all immuno assays
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Package form
Kit
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Antigen
Biotin
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Reacts with
Biotin
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Tested applications
The Polystreptavidin R Coating Kit is used for adsorptive coating of microplates, beads, membranes etc. made of metal or plastics like Polystyrene, Polyethylene, Polypropylene, Polycarbonate, Poly(methyl methacry-late) PMMA or Polyamides with Polystreptavidin “Maximum Capacity”.
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Specificity
Biotin
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Crossreactivity
N/A
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Preservative materials
If applicable mentioned in the product data sheet
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Storage recommendations
12 month
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Shipping and storage conditions
ice pack to temperature >25°C
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Long term storage
2-8°C
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Assay principle
adsorptive coating of Polystreptavidin
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Included in the standard package
PreCoating Solution 1 Concentrate, Dilution buffer for PreCoating Solution 1 Concentrate, Polystreptavidin R Concentrate, Dilution buffer for Polystreptavidin R Concentrate
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Not included in the standard package
Pipettes or pipetting equipment, vials for dilution, distilled or deionised water
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Samples to be analyzed
microplates, beads, membranes etc. made of metal, Nitrocellulose, ceramics or plastics like Polystyrene, Polyethylene, Polypropylene, Polycarbonate, Poly(methyl methacrylate) PMMA or Polyamides.
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PubMed citations
See the datasheet
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Technical datasheet
Contact us to receive the datasheet
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Representative figures
No Available
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Warnings
For Research Use only.
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