Apo-D, human recombinant
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Catalog number4326-1000
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PricePlease ask
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Size1 mg
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SynonymsApolipoprotein D, Apo-D, ApoD
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Alternative_namesApolipoprotein D, Apo-D, Apolipoprotein, apolipoproteins, ApoD
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DescriptionA protein involved in lipoprotein metabolism
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RecombinantYes
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SourceE. coli
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Purity by SDS PAGE≥95%
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AssaySDS-PAGE
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Purity≥95%
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Molecular Weight19.8 kDa
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Storage Temp-20°C
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ShippingGel pack
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Shelf Life12 months
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AppearanceLyophilized protein
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Physical form descriptionLyophilized from 1 mg/ml in 4 mM KH2PO4, 16 mM Na2HPO4 and 115 mM NaCl pH7.5.
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Reconstitution InstructionsReconstitute in dH₂O to a working volume of 0.5 mg/ml and let the lyophilized pellet dissolve completely. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use
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Background InformationApolipoprotein-D (Apo-D) is mainly associated with high density lipoproteins in human plasma. It is expressed in numerous tissues having high levels of expression in spleen, testes and brain. Apo-D is a multi-ligand, multi-functional transporter and transports a ligand from 1 cell to another within an organ, scavenge a ligand within an organ for transport to the blood or could transport a ligand from the circulation to specific cells within a tissue. The recombinant human Apo-D expressed from E.Coli is a single, non-glycosylated, Polypeptide chain containing 174 amino acids and having a molecular mass of 19.8 kDa.
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Short nameApo-D, recombinant
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHuman, Humans
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Alternative nameApo-D, H. sapiens Rec.
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Alternative techniquerec
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MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data