HPV33 E7 Antibody, Biotin Conjugated
#
-
Catalog numberbs-2969R-Biotin
-
Price:
-
Size0.1ml
-
-
Long nameHPV33 E7 Polyclonal Antibody, Biotin Conjugated
-
CategoryConjugated Primary Antibodies
-
ConjugationBiotin
-
Host OrganismRabbit (Oryctolagus cuniculus)
-
Target AntigenHPV33 E7
-
SpecificityThis is a highly specific antibody against HPV33 E7. This antibody will also detect E7 in HPV58, but with slightly less affinity, due to a one amino acid change in the immunogen sequence range.
-
ModificationUnmodified
-
Modification siteNone
-
ClonalityPolyclonal
-
ClonePolyclonal antibody
-
Concentration1ug per 1ul
-
Immunogen_range30-80/97
-
Subcellular locationNucleus
-
SourceKLH conjugated synthetic peptide derived from HPV33 E7
-
Tested applicationsWB, IHC-P
-
Recommended dilutionsWB(1:100-1000), IHC-P(1:100-500)
-
CrossreactivityVirus
-
Crossreactive species detailsHPV33
-
Antigen backgroundE7 protein has both transforming and trans-activating activities. Disrupts the function of host retinoblastoma protein RB1/pRb, which is a key regulator of the cell cycle. Induces the disassembly of the E2F1 transcription factors from RB1, with subsequent transcriptional activation of E2F1-regulated S-phase genes. Inactivation of the ability of RB1 to arrest the cell cycle is critical for cellular transformation, uncontrolled cellular growth and proliferation induced by viral infection. Stimulation of progression from G1 to S phase allows the virus to efficiently use the cellular DNA replicating machinery to achieve viral genome replication. Interferes with histone deacetylation mediated by HDAC1 and HDAC2, leading to activation of transcription (By similarity).
-
Purification methodThis antibody was purified via Protein A.
-
Storage conditionsKeep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
-
Excitation EmissionN/A
-
SynonymsE7 protein [Human papillomavirus type 33]; Human Papilloma Virus; Human papillomavirus type 33; Human papillomavirus type 33; Protein 33; early protein E7 [Human papillomavirus type 33].
-
PropertiesIf you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
-
Additional conjugationBiotinylated
-
French translationanticorps
-
Gene target
-
Short nameAnti-HPV33 E7
-
TechniqueAntibody, antibodies against human proteins, antibodies for, antibody Conjugates
-
IsotypeImmunoglobulin G (IgG)
-
LabelBiotin
-
Alternative nameAnti-HPV33 E7 PAb Biotin
-
Alternative techniqueantibodies
-
MeSH Data
-
Name
-
ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data