Goat anti Human Kappa Chain (FITC)
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Catalog number
MBS536347
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Price
Please ask
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Size
1 mg
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Products_type
Secondary Antibody
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Products_short_name
[Kappa Chain]
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Products_name_syn
[Goat anti Human Kappa Chain secondary (FITC)]
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Reactivity
Human kappa chain
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Specificity
Human kappa chain
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Purity
Goat anti Human Kappa Chain (FITC) was purified by affinity chromatography.
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Form
Lyophilized from 0.125M Na3B4O7, pH 8.0, with 0.075M NaCl, 0.005M EDTA, 10 mg/ml BSA, and 0.01% NaN3. Immunoglobulin and Protease free.
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Storage_stability
Store at 4 degree C until reconstitution. Following reconstitution aliquot and freeze at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
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Tested_application
ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
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Properties
This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Conjugation
Anti-FITC Antibody
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Latin name
Capra aegagrus hircus
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Gene target
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Gene symbol
PPM1K
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Short name
Goat anti Kappa Chain (FITC)
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Technique
anti human, anti, FITC, Goat, antibody to, anti human antibodies, Fluorescein, goats
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Host
Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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Label
FITC
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Species
Human, Goats, Humans
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Alternative name
caprine antibody to H. sapiens Kappa epitope (fluorecein)
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Alternative technique
antibodies, caprine, fluorescine
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Gene info
MeSH Data
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Name
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Concept
Scope note:
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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