Smad3 Antibody (pSer425): FITC

• Catalog number
  SPC-1427D-FITC
• Price
  Please ask
• Size
  100 µl

• Target
  Smad3 (pSer425)
• Conjugate
  FITC
• Research Area s
  Cell Signaling, Epigenetics and Nuclear Signaling, Nuclear Signaling Pathways, SMADs, Stem Cells, Signaling Pathways, TGF Beta, Cytoplasmic, Cancer, Cancer Metabolism, Response to Hypoxia, Cell Signaling, Metabolism, Metabolism processes, Hypoxia
• Alternative Name s
  Smad3 Antibody, Mothers against decapentaplegic homolog 3 Antibody, Mad3 Antibody, SMAD3_HUMAN Antibody, DKFZp686J10186 Antibody, SMAD Antibody, HSPC193 Antibody, SMAD family member 3 Antibody, hMAD-3 Antibody, MGC60396 Antibody, MAD homolog 3 Antibody, HST17436 Antibody, JV15-2 Antibody, MAD (mothers against decapentaplegic Drosophila) homolog 3 Antibody, JV152 Antibody, Mad homolog JV15 2 Antibody, SMAD 3 Antibody, JV15 2 Antibody, DKFZP586N0721 Antibody, MADH3 Antibody, MAD, Mothers against DPP homolog 3 Antibody, hSMAD3 Antibody, MADH 3 Antibody, LDS3 Antibody, Mad protein homolog Antibody, SMA and MAD related protein 3 Antibody, LDS1C Antibody, hMAD 3 Antibody
• Category
  Antibodies
• Product Type
  Polyclonal
• Immunogen
  Synthetic phospho-peptide from human Smad3 around the phosphorylation site of serine 425 (pSer425).
• Immunogen Species
  Human
• Accession Number
  NP_001138574.1
• Gene ID
  4088
• Swiss Prot
  P84022
• Applications
  WB, IHC
• Host Species
  Rabbit
• Species Reactivity
  Human, Mouse, Rat
• Antibody Dilution
  WB (1:250-1:1000); IHC (1:25-1:100); optimal dilutions for assays should be determined by the user.
• Purification
  Peptide Affinity Purified
• Storage Buffer
  PBS, pH 7.4, 150mM NaCl, 50% glycerol, 0.02% sodium azide
• Specificity
  Detects endogenous levels of Smad3 only when phosphorylated at serine 425 (pSer425). Detects a band at ~58 kDa.
• Storage Temperature
  -20ºC
• Shipping Temperature
  Blue Ice or 4ºC
• Cellular Localization
  Nucleus, Cytoplasm, Cytosol, Nuclear Chromatin, Nuclear Inner Membrane, Nucleoplasm, Plasma Membrane, Receptor Complex
• Field of Use
  For in vitro research use only.
• Technical data
• SDS
• Gross Weight kg
  1.4
• Net Weight g
  0.1
• Properties
  If you buy Antibodies supplied by StressMarq they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This StressMarq Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. StressMarq FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody
• French translation
  anticorps

• Gene target
  Smad3   pSer425  
• Short name
  Smad3 Antibody (pSer425): FITC
• Technique
  Antibody, FITC, antibodies against human proteins, antibodies for, Fluorescein
• Isotype
  IgG
• Label
  FITC
• Alternative name
  SMAD family member 3 (antibody to-) (pSer425): fluorecein
• Alternative technique
  antibodies, fluorescine
• Alternative to gene target
  SMAD family member 3, HSPC193 and HsT17436 and JV15-2 and LDS1C and LDS3 and MADH3, SMAD3 and IDBG-18366 and ENSG00000166949 and 4088, transforming growth factor beta receptor, nuclei, Smad3 and IDBG-175693 and ENSMUSG00000032402 and 17127, SMAD3 and IDBG-645466 and ENSBTAG00000012599 and 515125

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Similar products