Fumarate Colorimetric Assay Kit
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Catalog number
K633-100
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Price
Please ask
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Size
100 assays
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Summary
• Detection method- Absorbance (450 nm) • Species reactivity- Mammalian • Application- Kit can detect as low as 1 nmol of fumarate per well (20 µM).
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Sample Type
Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids
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Features Benefits
• Simple procedure; takes ~ 40 minutes • Fast and convenient
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Storage Conditions
-20°C
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Shipping Conditions
gel pack
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Shelf life
12 months
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Background
Fumarate (HO₂CCH=CHCO₂H-) is an intermediate in the Kreb’s cycle used by cells to metabolize food to form ATP. In the mammalian liver, Fumarate is also a product of the Urea cycle where its release in the cytosol leads to its conversion into malate and subsequently oxaloacetate while generating NADH in the cytosol. The human skin naturally produces fumaric acid when exposed to sunlight. In fact, fumaric acid esters have been used to treat psoriasis, possibly due to an impaired production of fumaric acid in the skin. Fumaric acid has also been used in beverages, baking powders and candy. BioVision’s Fumarate Assay Kit provides a convenient tool for sensitive detection of the fumarate in a variety of samples. The fumarate Enzyme Mix recognizes fumarate as a specific substrate leading to proportional color development. The amount of fumarate can therefore be easily quantified using a colorimetric assay (λ = 450 nm). It can detect as low as 1 nmol of fumarate per well (20 µM).
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Test
Biovision supplies other types of Assays as 1.
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Properties
Colorimetric assays or detection use UV absorption or enzymatic color reaction.
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Gene target
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Short name
Fumarate Colorimetric Assay Kit
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Technique
colorimetric, Assay, assays, colorimetry
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Host
Assay
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Alternative name
Fumarate colorimetry test reagent
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Alternative technique
assays, arrays, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Product images
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