Cell-Mediated Cytotoxicity Fluorometric Assay Kit (7-AAD/CFSE)
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Catalog numberK315-100
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PricePlease ask
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Size100 assays
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DescriptionDirect assay to measure cell-mediated cytotoxicity,
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Summary• Detection method- Fluorescence (FL1 channel and FL3 channel) • Applications- Measurement of cell cytotoxicity in response to drug or toxin treatment • Quantification of the cytotoxic effect of immune effector cells on target cells • Assessment of physiological mediators and antibodies that affect cell cytotoxicity
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Detection MethodFluorescence (FL1 channel and FL3 channel)
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Applications• Measurement of cell cytotoxicity in response to drug or toxin treatment • Quantification of the cytotoxic effect of immune effector cells on target cells • Assessment of physiological mediators and antibodies that affect cell cytotoxicity
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Sample TypeAdherent and suspension cells
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Features Benefits• Direct method to measure cell-mediated cytotoxicity. • Does not require cell lysis. • Provides multi-parametric analysis.
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Storage Conditions-20°C
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Shipping Conditionsgel pack
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Shelf life12 months
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BackgroundCell-mediated cytotoxicity is an important phenomenon characterized by cytolysis of a compromised cell in the body by immune system. Activation of immune system leads to removal of target cells infected by pathogens or transformed cells/cancer cells. This process is mediated by antibody-dependent cell-mediated cytotoxicity (ADCC), complement-mediated cytotoxicity, or lymphocyte-mediated cytotoxicity. BioVision’s Cell-Mediated Cytotoxicity Assay Kit contains carboxyfluorosuccinimide ester (CFSE), a green fluorescent probe that labels live target cells and 7-aminoactinomycin D (7-AAD), a red fluorescent probe that labels late apoptotic and necrotic target cells killed in the cytotoxicity assay. This assay does not require cell lysis and provides a direct measurement of cytotoxicity instead of indirect indicators such as release of ATP or lactate dehydrogenase activity. The flow cytometry-based method provides robust data and enables multi-parametric analysis.
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TestBiovision supplies other types of Assays as 1.
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Additional descriptionFor cells, cell lines and tissues in culture till half confluency.
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Gene target
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Gene symbolPIRC34, PIRC33, PIRC32, SNORD114-7, IGHV3OR15-7, SNORD115-7, RNU6-7, PIRC29, PIRC30, PIRC31
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Short name-Mediated Cytotoxicity Fluorometric Assay Kit (7-AAD/CFSE)
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TechniqueAssay, assays
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HostAssay
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Alternative namecellular-Mediated Cytotoxicity fluorescence test reagent (7-AAD/CFSE)
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Alternative techniquearrays, kits
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Alternative to gene targetv-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Tissuecell
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Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 34
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 33
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 32
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 114-7
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Synonyms
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Locus
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Discovery year2006-09-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 3/OR15-7 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable 3/OR15-7
- immunoglobulin heavy variable 3/OR15-7 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-17
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Entrez gene record
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Classification
- Immunoglobulin heavy (IGH) orphons
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 115-7
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene nameRNA, U6 small nuclear 7
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Synonyms
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Locus
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Discovery year2008-06-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nuclear RNAs
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 29
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 30
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Locus
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Discovery year2009-11-05
-
Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 31
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters