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Human CellExp Biotinylated Fc human recombinant

Human CellExp Biotinylated Fc human recombinant is available 3 times from Biovision labs

7877-5 | Human CellExp Biotinylated PD-1 / PDCD1, His Tag & Fc Tag, human recombinant size: 5 μg | 397.11 USD

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P1004-20 | Human CellExp™ Biotinylated Fc gamma RI/CD64, human recombinant size: 20 μg | 291.59 USD

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P1004-100 | Human CellExp™ Biotinylated Fc gamma RI/CD64, human recombinant size: 100 μg | 1,056.31 USD

Source HEK 293 cells
Biological activity Immobilized Yervoy Ipilimumab (Human IgG1) at 10 μg/mL (100 μL/well) can bind Biotinylated Human Fc gamma RI with a linear range of 1.5-50 ng/ml.
Properties Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source Recombinants or rec. proteins
Group recombinants
Short name CellExp™ Biotinylated Fc gamma RI/CD64, recombinant
Species Humans
Alternative name H. sapiens CellExp™ Biotinylated fragment c g RI/CD64, H. sapiens recombinant
1. Gene info
Identity3613
GeneFCGR1A
Long gene name Fc fragment of IgG receptor Ia
Synonyms gene name
  • Fc fragment of IgG, high affinity Ia, receptor for (CD64)
  • Fc fragment of IgG, high affinity Ia, receptor (CD64)
Synonyms
GenBank acession
Locus1q21.2
Discovery year 1992-12-03
Entrez gene record2209
Pubmed identfication
RefSeq identity
Classification
  • CD molecules
  • Immunoglobulin like domain containing
Havana BLAST/BLATOTTHUMG00000012089
MeSH Data
Concept
Scope note:Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
Tree numbers
  • E01.370.225.500.363.342
  • E01.370.225.500.386.350
  • E05.196.712.516.600.240.350
  • E05.200.500.363.342
  • E05.200.500.386.350
  • E05.242.363.342
  • E05.242.386.350
Additional information
PubMedTLR4 is hyperexpressed in the intestinal mast cells of Crohn disease patients.
Data indicate that upon sialylation, the affinities for Fcgamma receptors are reduced, whereas those for alternative cellular receptors, such as dendritic cell-specific intercellular adhesion molecule-3-grabbing nonintegrin (DC-SIGN)/CD23, are increased.
Aspartic acid at position 265 of IgG is a residue critically implicated in triggering the Fc-associated effector functions of IgG, probably by defining a crucial three-dimensional structure of the Fc region.
Data suggest that flagellin-specific IgG/Fc gamma receptor I complex immune responses activate mast cells in the intestine and play important roles in the pathogenesis of intestinal immune inflammation.
Expression of TSLP receptor was upregulated on atopic DCs primed with H22-Fel d 1 through a pathway regulated by FcgammaRI-associated signaling components.
CD64 expression on neutrophils increases significantly in neonates with sepsis and is a useful diagnostic marker for early diagnosis of neonatal infection.
neutrophil CD64 expression tends to be low in the healthy state and increases in patients with advanced cancer even in the absence of active infection
Our findings demonstrate that remarkable CD64 expression during Kawasaki dsease flare-ups may serve as a biomarker for diagnosis.
This study demonstrated that CD64 discriminates between critically ill patients with culture positive and negative sepsis and correlates with severity of disease. However, CD64 index is not a good predictor for 28-day mortality in the critically ill patient.
CD64 seems to be a promising marker of infection in the intensive care setting, with Leuko64 showing a slight advantage
Product images
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Target Fc gamma RI
Background Receptors that recognize the Fc portion of IgG are divided into three groups designated Fc gamma RI, RII, and RIII, also known respectively as CD64, CD32, and CD16. Fc gamma RI binds IgG with high affinity and functions during early immune responses. Fc gamma RII and RIII are low affinity receptors that recognize IgG as aggregates surrounding multivalent antigens during late immune responses.
Concentration N/A
Molecular weight The protein has a calculated MW of 34.3 kDa. The reducing (R) protein migrates as 45-60 kDa in SDS-PAGE due to glycosylation.
Synonym High affinity immunoglobulin gamma Fc receptor I, IgG Fc receptor I, Fc-gamma RI, FcRI, Fc-gamma RIA, FcgammaRIa, CD64, FCGR1A
Other name High affinity immunoglobulin gamma Fc receptor I, IgG Fc receptor I, Fc-gamma RI, FcRI, Fc-gamma RIA, FcgammaRIa, CD64 ,FCGR1A
NCBI gene number 2209
NCBI gene FCGR1A
Gene source Human
NCBI number P12314
Recombinant Yes
Purification ≥92%
Tested applications SDS-PAGE, N/A
Tested activity Fc gamma RI binding ability is measured by functional ELISA.
Reesults N/A
Binding ability Immobilized Yervoy Ipilimumab (Human IgG1) at 10 μg/mL (100 μL/well) can bind Biotinylated Human Fc gamma RI with a linear range of 1.5-50 ng/ml.
Unit N/A
Storage condition -20°C
Shipping under Gel pack
Physical appearance Dry powder
Physical properties Lyophilized powder
How to reconstitute Reconstitute in sterile deionized water to a stock solution of 200 µg/mL. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% (W/V) HSA or BSA) is recommended for further dilution and long term storage.
Aa sequence Gln 16 – Pro 288
Before use Centrifuge the vial prior to opening.
Notes For Research Use Only! Not to be used in humans
Synonyms PDCD1, PD1, CD279, SLEB2, hPD-1, hPD-l
Alternates names PDCD1, PD1, CD279, SLEB2, hPD-1, hPD-l
Taglines Inhibits the T-cell proliferation and production of IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway.
Biomolecule/Target PD-1/PDCD1
NCBI Gene ID #. 5133
NCBI Gene Symbol PDCD1
Gene Source Human
Accession # Q15116
Purity N/A
Activity (Specifications/test method) N/A
Results Measured by its binding ability in a functional ELISA. Immobilized Human PD-L1, Fc Tag at 5 μg/mL (100 µl/well), can bind Biotinylated Human PD-1, His Tag & Fc Tag with a linear range of 0.01-0.1 μg/mL.
Molecular Weight Biotinylated rhPDCD1 Fc Chimera, fused with Fc fragment of human IgG1 at the C-terminus, has a calculated MW of 42.6 kDa. The predicted N-terminus is Pro 21. DTT-reduced Protein migrates as 50 kDa due to glycosylation. The primary amine of biotinylated rhPDCD1 Fc Chimera was labeled by biotin for detection in ELISA, dot blot or western blot using streptavidin or avidin-conjugated probes.
Storage Temp. -20°C
Shipping Gel Pack
Shelf Life 1 year
Appearance Lyophilized
Physical form description Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
Reconstitution Instructions Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Handling Centrifuge the vial prior to opening.
Usage For Research Use Only! Not to be used in humans
Technique Recombinant, FC
Label His
Alternative technique rec
Alternative to gene target programmed cell death 1, CD279 and hPD-1 and hPD-l and hSLE1 and PD-1 and PD1 and SLEB2, PDCD1 and IDBG-85346 and ENSG00000188389 and 5133, protein binding, Cell surfaces, Pdcd1 and IDBG-184148 and ENSMUSG00000026285 and 18566, PDCD1 and IDBG-642702 and ENSBTAG00000011543 and 613842
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