anti- SKP2 antibody

• Catalog number
  FNab07898
• Price
  Please ask
• Size
  2X100μg

• Form
  liquid
• Purification
  Immunogen affinity purified
• Purity
  ≥95% as determined by SDS-PAGE
• Storage
  PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
• Background
  This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class; in addition to an F-box, this protein contains 10 tandem leucine-rich repeats. This protein is an essential element of the cyclin A-CDK2 S-phase kinase. It specifically recognizes phosphorylated cyclin-dependent kinase inhibitor 1B (CDKN1B, also referred to as p27 or KIP1) predominantly in S phase and interacts with S-phase kinase-associated protein 1 (SKP1 or p19). In addition, this gene is established as a protooncogene causally involved in the pathogenesis of lymphomas. Alternative splicing of this gene generates three transcript variants encoding different isoforms.
• Immunogen
  S-phase kinase-associated protein 2 (p45)
• Synonyms
  FBXL1
• Calculated MW
  Refer to figures
• Uniprot ID
  Q13309
• Specificity
  Human, Mouse
• Tested Application
  ELISA, WB, IHC
• Recommended dilution
  WB: 1:500 - 1:2000; IHC: 1:50 - 1:200
• Image1
  Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using FNab07898( SKP2 Antibody) at dilution of 1:200 heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
• Image4
  HEK-293 cells were subjected to SDS PAGE followed by western blot with FNab07898( SKP2 Antibody) at dilution of 1:1000
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
• Properties
  If you buy Antibodies supplied by FineTest they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  SKP2  
• Gene symbol
  SKP2
• Short name
  anti- SKP2 antibody
• Technique
  Antibody, anti, antibody to, antibodies against human proteins, antibodies for
• Host
  Rabbit
• Isotype
  IgG
• Alternative name
  antibody to- SKP2 (antibody to-)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:10901
• Gene
  SKP2
• Long gene name
  S-phase kinase associated protein 2
• Synonyms gene name
  • S-phase kinase-associated protein 2 (p45)
  • S-phase kinase-associated protein 2, E3 ubiquitin protein ligase
• Synonyms
  • FBXL1
  • FBL1
  • p45
• GenBank acession
  • U33761
• Locus
  5p13.2
• Discovery year
  1995-12-18
• Entrez gene record
  6502
• Pubmed identfication
  • 8646875
• RefSeq identity
  • NM_005983
• Classification
  • F-box and leucine rich repeat proteins
• VEGA ID
  OTTHUMG00000131106

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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