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Antibody Target
Clusterin
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Target Alternative Name
TRPM-2, Apolipoprotein J, APO-J, CLI, CLU, SGP-2, AAG4, KUB1, SGP2, SP-40, TRPM2, mgC24903.
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Antibody Type
Polyclonal
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Species Reactivities
Human.
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Immunogen Sequence
This CLU antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 71-99 amino acids from the N-terminal region of human CLU.
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Accession
P10909
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Gene Id
1191
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Appearance
Colorless liquid
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Form
Liquid
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Formulation
100 µl of antibody in PBS with 0.09% (W/V) sodium azide
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Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
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Application
Western blot, IHC, FACS.
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Positive Control
Western blot: A549 cell lysate, IHC: Human brain tissue, FACS: A549 cells.
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Application And Usages
Western blot: ~1:1000, IHC: ~1:50-1:100, FACS: ~1:10–1:50.
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Description
Native Apolipoprotein J (ApoJ), also named Clusterin, is a heavily glycosylated, 75-80 kDa disulfide-linked heterodimeric protein. Despite being cloned since 1989, no genuine function has been attributed to ApoJ so far. The protein has been reportedly implicated in several diverse physiological processes such as sperm maturation, lipid transportation, complement inhibition, tissue remodeling, membrane recycling, cell-cell and cell-substratum interactions, stabilization of stressed proteins in a folding-competent state and promotion or inhibition of apoptosis. ApoJ gene is differentially regulated by cytokines, growth factors and stress-inducing agents. Clusterin is up- or down regulated on the mRNA or protein level in many pathological and clinically relevant situations including cancer, organ regeneration, infection, Alzheimer disease, retinitis pigmentosa, myocardial infarction, renal tubular damage, autoimmunity and others.
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Storage Condition
-20 °C
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Shipping Condition
gel pack
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Shelf Life
12 months
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Usage
For Research Use Only! Not to be used in humans.
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Handling
The antibody solution should be gently mixed before use.
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Western Blot Verified
Verified
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Immunocytochemistry Verified
Verified
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Immunofluorescence Verified
Not Verified
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Immunoprecipitation Verified
Not Verified
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ELISA Verified
Not Verified
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ChIP Verified
Not Verified
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Flow Cytometry Verified
Verified
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Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
