ApoJ/Clusterin, human recombinant
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Catalog number7584-10
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Price:
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Size10 ug
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SynonymsCLI, AAG4, KUB1, SGP2, SGP-2, SP-40, TRPM2, MGC24903, Clusterin, Apolipoprotein J, ApoJ
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Alternative_namesCLI, AAG4, KUB1, SGP2, SGP-2, SP-40, TRPM2, MGC24903, Clusterin, Apolipoprotein J, ApoJ
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DescriptionA heavily glycosylated protein upregulated in multiple pathological conditions
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RecombinantYes
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SourceE. coli
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Purity by SDS PAGE≥90%
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AssaySDS-PAGE
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Activity Specifications test methodThe chaperone activity of ApoJ was confirmed by its ability to bind to proteins under physical stress such as heat (e.g. Thyroglobulin) and chemical stress such as change in pH (e.g. Procathepsin K).
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Biological activityThe chaperone activity of ApoJ was confirmed by its ability to bind to proteins under physical stress such as heat (e.g. Thyroglobulin) and chemical stress such as change in pH (e.g. Procathepsin K).
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Molecular Weight51.1 kDa (23-449 aa with a C-terminal His-tag)
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Storage Temp-20°C
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ShippingGel Pack
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Shelf Life12 months
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AppearanceLyophilized
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Physical form descriptionLyophilized from 2 mg/ml solution of ApoJ in 25 mM Na2HPO4 and 100 mM NaCl (pH 7.5).
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Reconstitution InstructionsReconstitute in dH2O to a working concentration of 0.5 mg/ml and let the lyophilized pellet dissolve completely.
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Background InformationNative Apolipoprotein J (ApoJ), also named Clusterin, is a heavily glycosylated, 75-80 kDa disulfide-linked heterodimeric protein. Despite being cloned since 1989, no genuine function has been attributed to ApoJ so far. The protein has been reportedly implicated in several diverse physiological processes such as sperm maturation, lipid transportation, complement inhibition, tissue remodeling, membrane recycling, cell-cell and cell-substratum interactions, stabilization of stressed proteins in a folding-competent state and promotion or inhibition of apoptosis. ApoJ gene is differentially regulated by cytokines, growth factors and stress-inducing agents. Clusterin is up- or down regulated on the mRNA or protein level in many pathological and clinically relevant situations including cancer, organ regeneration, infection, Alzheimer disease, retinitis pigmentosa, myocardial infarction, renal tubular damage, autoimmunity and others.
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolCLU
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Short nameApoJ/Clusterin, recombinant
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHuman, Humans
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Alternative nameApoJ/Clusterin, H. sapiens Rec.
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Alternative techniquerec
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Gene info
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Identity
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Gene
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Long gene nameclusterin
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Synonyms gene
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Synonyms gene name
- clusterin (complement lysis inhibitor, SP-40,40, sulfated glycoprotein 2, testosterone-repressed prostate message 2, apolipoprotein J)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1990-07-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Complement system regulators and receptors
- MicroRNA protein coding host genes
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data