HSP27 (phospho-S15) polyclonal antibody
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Catalog numberAP0245-50ul
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Price:
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Size50ul
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Alternative NameHeat shock protein beta-1; HspB1; 28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; HSP 27; Stress-responsive protein 27; SRP27; HSPB1; HSP27; HSP28
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ImmunogenSynthetic phosphopeptide derived from human HSP27 around the phosphorylation site of Serine 15.
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Swiss ProtP04792
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ReactivityHuman,Mouse,Rat
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ApplicationsWB IHC IP
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Application_allWB: 1:500~1:1000 IHC: 1:50~1:200 IP: 1:10~1:100
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ProductRabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
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Purification PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
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Storage StabilityStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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SpecificityHSP27 (phospho-S15) polyclonal antibody detects endogenous levels of HSP27 protein only when phosphorylated at Ser15
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UsageWestern blot (WB) analysis of HSP27 (phospho-S15) pAb at 1:500 dilution Lane1:L02 whole cell lysate Lane2:SGC7901 whole cell lysate Lane3:HEK293T transfected with pCDNA3.1-Flag-HSP27 whole cell lysate Lane4:HEK293T whole cell lysate
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BiowMW~ 27 kDa
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NoteFor research use only, not for use in diagnostic procedure.
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PropertiesIf you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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French translationanticorps
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Gene target
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Gene symbolRPS15, HSPB1
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Short nameHSP27 (phospho-S15) polyclonal antibody
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TechniquePolyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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HostRabbit
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Alternative nameHSP27 (phosphorilated-S15) polyclonal (antibody to-)
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Alternative techniquepolyclonals, antibodies
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Gene info
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Identity
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Gene
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Long gene nameribosomal protein S15
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Synonyms
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Synonyms name
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Locus
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Discovery year1992-04-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- S ribosomal proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameheat shock protein family B (small) member 1
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Synonyms gene name
- heat shock 27kD protein 1
- heat shock 27kDa protein 1
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Synonyms
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GenBank acession
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Locus
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Discovery year1991-07-09
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Entrez gene record
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Pubmed identfication
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Classification
- Small heat shock proteins
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data