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Availability
Please contact us to check the availability
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Immunogen
Helicobacter ATTC-strain 43504, whole cell lysate
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Specificity
Helicobacter pylori (eventually also other subspecies)
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Species reactivity
Helicobacter
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Product presentation
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. Gentaur Art .No. PU002.
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Product category
Primary Antibody
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Antibody s raised in
Rabbit
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Antibody s isotype
Ig
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Antibody s clone
polyclonal
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Product is supplied as
liquid concentrate
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Recommended concentration for use
1:10-1:50
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Protein concentration
50 µg/ml
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Positive control
Ulcus duodeni or Ulcus gastricus
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Pretreatment
Pre-treatment with pronase 0,1% (Code no. DE110) or unmasking fluid G (Code No. DE007)
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Tested applications
IHC(C, P)
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Recommended secondary reagent
We recommend the use of Gentaur's Universal Staining System DAB (Art. No. DA005) or AEC (Art. No. AE005), for higher sensitivities VECTASTAIN Elite ABC (Art. No. PK-6101) or ABC AP (Art. No. AK-5001) systems are applicable.
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Storage method
Store at 2-8°C
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UniProt number
Go to UniProt/SwissProt
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Litterature
1. Dooley C.P., Cohen H., Fitzgibbons P.L., Bauer M., Appleman M.D., Perez-Perez G.I. et al. (1989) Prevalence of Helicobacter pylori infection and histologic gastritis in asymptomatic persons. N. Engl. J. Med. 321; 1562-1566. 2. Parsonnet J., Friedman G.D., Vandersteen D.P., Chang Y., Vogelman J.H., Orentreich N., et al. (1991) Helicobacter pylori infection and the risk of gastric carcinoma. N. Engl. J. Med. 325; 1127-1131 3. Nomura A., Stemmerman G.N., Chyou P.-H. Kato I., Perez-Perez G.I., Blaser M.J. (1991) Helicobacter pylori infection and gastritic carcinoma among Japanese Americans in Hawaii. N. Engl. J. Med. 325; 1132-1136. 4. Kreuning J., Lindeman J., Biemond I., and Lamers C.B.H.W. (1994) Relation between IgG and IgA antibody titres against Helicobacter pylori in serum and severity of gastritis in asymptomatic subjects. J. Clin. Pathol. 47: 227-231.
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Tips
*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by bio logo. Rabbit antibodies are very stable and can be stored for several days at room temperature. bio logo adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
