B. subtilis Recombinant, Oxalate oxidase (OxOx)

• Catalog number
  P1091-100
• Price
  Please ask
• Size
  100 ug

• Synonyms
  OxO, OxOx, OxO_r
• Alternative_names
  OxO, OxOx, OxO_r
• Description
  Converts oxalate to formate and CO2 in an O(2)-dependent reaction
• Recombinant
  Yes
• Source
  E. Coli
• Purity by SDS PAGE
  ≥90%
• Assay
  SDS-PAGE
• Activity Specifications test method
  Measured by the conversion of 1 µmole of oxalate to CO2 and H2O2 per minute at pH 4.5 and 25C.
• Biological activity
  ≥ 230 mU/mg and is defined as the amount of enzyme that catayze the conversion of converts 1 µmole of oxalate to CO2 and H2O2 per minute at pH 4.5 and 25C.
• Results
  ≥ 230 mU/mg
• Unit Definition
  One unit is the amount of enzyme that converts 1 µmole of oxalate to CO2 and H2O2 per minute at pH 4.5 and 25C.
• Molecular Weight
  43.6 kDa
• Storage Temp
  -20°C
• Shipping
  Gel pack
• Shelf Life
  12 months
• Appearance
  Freeze dried
• Physical form description
  Freeze-dried from proprietary buffer
• Reconstitution Instructions
  Reconstitute to 2 mg/mL in sterile water
• Background Information
  Oxalate oxidase is a member of the cupin superfamily. It catalyzes the breakdown of oxalate to hydrogen peroxide and carbon dioxide. Oxalate oxidase and oxalate decarboxylase are two similar enzymes that share similar structural and sequence identity. Both enzymes use oxalate as substrate and manganese ions as cofactors. BioVision’s oxalate oxidase is derived from B. subtilis oxalate decarboxylase by introducing three mutations: S161D, E162A and N163S in the active-site loop.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  subtilis   Oxalate   oxidase   OxOx  
• Short name
  B. subtilis Recombinant, Oxalate oxidase (OxOx)
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Alternative name
  B. subtilis Rec., Oxalate oxidase (OxOx)
• Alternative technique
  rec

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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