NME3, human recombinant

• Catalog number
  7825-100
• Price
  Please ask
• Size
  100 ug

• Synonyms
  Nucleoside diphosphate kinase 3, c371H6.2, DR-nm23, KIAA0516, NDPK-C, NDPKC, NM23-H3, NM23H3.
• Alternative_names
  Nucleoside diphosphate kinase 3, c371H6.2, DR-nm23, KIAA0516, NDPK-C, NDPKC, NM23-H3, NM23H3.
• Description
  Important for the synthesis of nucleoside triphosphates and may play a role in apoptosis induction and hematopoiesis.
• Recombinant
  Yes
• Source
  E. coli.
• Purity by SDS PAGE
  ≥95%
• Assay
  SDS-PAGE
• Biological activity
  Specific activity is > 20 units/ml, in which one unit will convert 1.0 µmole each of TDP and ATP to TTP and ADP per minute at pH 7.6 at 25ºC in a coupled system with PK/LDH.
• Results
  Specific activity is > 20 units/ml
• Unit Definition
  One unit will convert 1.0 µmole each of TDP and ATP to TTP and ADP per minute at pH 7.6 at 25ºC in a coupled system with PK/LDH.
• Molecular Weight
  19.1 kDa (169 aa, 22-169 aa + His Tag)
• Storage Temp
  -20°C
• Shipping
  gel pack
• Shelf Life
  12 months
• Concentration
  0.5 mg/ml
• Appearance
  Liquid
• Physical form description
  0.5 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 50% glycerol, 0.1 M NaCl and 2 mM DTT
• Background Information
  NME3, also known as, a potential suppressor of metastasis, is expressed at a much lower level in highly metastatic cells than in cells with lower metastatic potential. It is important for the synthesis of nucleoside triphosphates and may play a role in apoptosis induction and hematopoiesis. It is preferentially expressed during early stages of myeloid differentiation of highly purified CD34+ cells. Recombinant human NME3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
• Amino acid sequence
  MGSSHHHHHH SSGLVPRGSH MERTFLAVKP DGVQRRLVGE IVRRFERKGF KLVALKLVQA SEELLREHYA ELRERPFYGR LVKYMASGPV VAMVWQGLDV VRTSRALIGA TNPADAPPGT IRGDFCIEVG KNLIHGSDSV ESARREIALW FRADELLCWE DSAGHWLYE
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  NME3  
• Gene symbol
  NME3
• Short name
  NME3, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  NME/NM23 nucleoside diphosphate kinase 3, H. sapiens Rec.
• Alternative technique
  rec
• Alternative to gene target
  NME/NM23 nucleoside diphosphate kinase 3, c371H6.2 and DR-nm23 and NDPK-C and NDPKC and NM23-H3 and NM23H3, NME3 and IDBG-9255 and ENSG00000103024 and 4832, metal ion binding, multiple, Nme3 and IDBG-149843 and ENSMUSG00000073435 and 79059, NME3 and IDBG-634801 and ENSBTAG00000016552 and 515663

Gene info

• Identity
  HGNC:7851
• Gene
  NME3
• Long gene name
  NME/NM23 nucleoside diphosphate kinase 3
• Synonyms gene name
  • non-metastatic cells 3, protein expressed in
• Synonyms
  • DR-nm23
  • NM23-H3
  • NDPKC
• GenBank acession
  • U29656
• Locus
  16p13.3
• Discovery year
  1996-12-19
• Entrez gene record
  4832
• Pubmed identfication
  • 9067290
  • 19852809
• RefSeq identity
  • NM_002513
• Classification
  • NME/NM23 family
• VEGA ID
  OTTHUMG00000128635

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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