Anti-KI67 Rabbit Monoclonal Antibody
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Catalog number
A1135-100
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Price
Please ask
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Size
100 µl
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Antibody Target
MKI67
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Target Alternative Name
KIA, KI-67, MKI67
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Antibody Type
Monoclonal
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Clone
RM227
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Species Reactivities
Human and mouse
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Immunogen Sequence
A peptide corresponding to the internal region of human Ki67
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Accession
P46013
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Gene Id
4288
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Appearance
Colorless liquid
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Form
Liquid
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Formulation
In 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
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Purification
Affinity purified using protein A
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Application
IHC, WB
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Positive Control
WB: HeLa and K562 cell lysate; IHC: human breast cancer and tonsil tissue
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Application And Usages
IHC: 1:100 - 1:400 dilution ; WB: 1:100 - 1:500 dilution
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Description
Ki-67 is a nuclear protein that is expressed in proliferating cells and may be required for maintaining cell proliferation. Ki-67 has been used as a marker for cell proliferation of solid tumors and some hematological malignancies. A correlation has been demonstrated between Ki-67 index and the histopathological grade of neoplasms. Assessment of Ki-67 expression in renal and ureter tumors shows a correlation between tumor proliferation and disease progression, thus making it possible to differentiate high-risk patients. Ki-67 expression may also prove to be important for distinguishing between malignant and benign peripheral nerve sheath tumors.
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Storage Condition
-20 °C
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Shipping Condition
gel pack
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Shelf Life
12 months
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Usage
For Research Use Only! Not to be used in humans.
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Handling
The antibody solution should be gently mixed before use.
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Western Blot Verified
Verified
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Immunocytochemistry Verified
Verified
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Immunofluorescence Verified
Not Verified
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Immunoprecipitation Verified
Not Verified
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FACS Verified
Not Verified
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ELISA Verified
Not Verified
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ChIP Verified
Not Verified
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Dot Blot Verified
Not Verified
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Flow Cytometry Verified
Not Verified
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Additional description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rabbits are used for polyclonal antibody production by Biovision. Rabbit antibodies are very stable and can be stored for several days at room temperature. Biovision adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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French translation
anticorps
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Gene target
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Short name
Anti-KI67 Rabbit Monoclonal Antibody
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Technique
Antibody, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies
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Host
Rabbit, Rabbits
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Isotype
Rabbit IgG
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Alternative name
Antibody toKI67 production species: rabbit monoclonal (antibody to-)
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Alternative technique
antibodies, rabbit-anti
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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