Human CellExp™ ECE1, Human Recombinant

• Catalog number
  P1227-50
• Price
  Please ask
• Size
  50 ug

• Synonyms
  Endothelin-converting enzyme 1, ECE-1
• Alternative_names
  Endothelin-converting enzyme 1, ECE-1
• Description
  Converts big endothelin-1 to endothelin-1
• Recombinant
  Yes
• Source
  HEK 293 cells
• Purity by SDS PAGE
  > 95%
• Assay
  SDS-PAGE
• Storage Temp
  -20°C
• Shipping
  Gel Pack
• Shelf Life
  12 months
• Appearance
  Lyophilized
• Physical form description
  Lyophilized from a 0.2 um filtered solution of PBS, pH7.4
• Reconstitution Instructions
  Reconstitute the lyophilized protein in ddH2O
• Background Information
  Endothelin-Converting Enzyme-1 (ECE-1) is a single-pass type II transmembrane (TM) protein with a short cytoplasmic tail and a large ectodomain. ECE-1 is a zinc protease of the neprilysin (NEP) family, which also includes ECE-2, PEX, XCE, DINE, and Kell, and several NEP-like proteins. It is widely expressed and has several alternatively spliced forms that differ in their TM domain or cytoplasmic tail. All isoforms of ECE-1 are expressed in umbilical vein endothelial cells, polynuclear neutrophils, fibroblasts, atrium cardiomyocytes and ventricles. Endothelin-converting enzyme-1 is involved in the proteolytic processing of Endothelin-1 (EDN1), Endothelin-2 (EDN2), and Endothelin-3 (EDN3) to biologically active peptides. Defects in ECE1 are a cause of Hirschsprung disease, cardiac defects and autonomic dysfunction (HSCRCDAD). It is a form of Hirschsprung disease with skip-lesions defects, craniofacial abnormalities and other dysmorphic features, and autonomic dysfunction.
• Amino acid sequence
  HHHHHHHHQYQTRSPSVCLSEACVSVTSSILSSMDPTVDPCHDFFSYACGGWIKANPVPDGHSRWGTFSNLWEHNQAIIKHLLENSTASVSEAERKAQVYYRACMNETRIEELRAKPLMELIERLGGWNITGPWAKDNFQDTLQVVTAHYRTSPFFSVYVSADSKNSNSNVIQVDQSGLGLPSRDYYLNKTENEKVLTGYLNYMVQLGKLLGGGDEEAIRPQMQQILDFETALANITIPQEKRRDEELIYHKVTAAELQTLAPAINWLPFLNTIFYPVEINESEPIVVYDKEYLEQISTLINTTDRCLLNNYMIWNLVRKTSSFLDQRFQDADEKFMEVMYGTKKTCLPRWKFCVSDTENNLGFALGPMFVKATFAEDSKSIATEIILEIKKAFEESLSTLKWMDEETRKSAKEKADAIYNMIGYPNFIMDPKELDKVFNDYTAVPDLYFENAMRFFNFSWRVTADQLRKAPNRDQWSMTPPMVNAYYSPTKNEIVFPAGILQAPFYTRSSPKALNFGGIGVVVGHELTHAFDDQGREYDKDGNLRPWWKNSSVEAFKRQTECMVEQYSNYSVNGEPVNGRHTLGENIADNGGLKAAYRAYQNWVKKNGAEHSLPTLGLTNNQLFFLGFAQVWCSVRTPESSHEGLITDPHSPSRFRVIGSLSNSKEFSEHFRCPPGSPMNPPHKCEVW
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  CellExp™   ECE1  
• Gene symbol
  ECE1, ECE1-AS1
• Short name
  CellExp™ ECE1, Recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Humans
• Alternative name
  H. sapiens CellExp™ endothelin converting enzyme 1, H. sapiens Rec.
• Alternative technique
  rec
• Alternative to gene target
  endothelin converting enzyme 1, ECE, ECE1 and IDBG-93094 and ENSG00000117298 and 1889, metal ion binding, Cell surfaces, Ece1 and IDBG-199213 and ENSMUSG00000057530 and 230857, ECE1 and IDBG-645619 and ENSBTAG00000002977 and 281133

Gene info

• Identity
  HGNC:3146
• Gene
  ECE1
• Long gene name
  endothelin converting enzyme 1
• Synonyms gene
  • ECE
• GenBank acession
  • D49471
• Locus
  1p36.12
• Discovery year
  1995-12-08
• Entrez gene record
  1889
• Pubmed identfication
  • 7805846
  • 7864876
  • 17592116
• RefSeq identity
  • NM_001397
• Classification
  • M13 metallopeptidases
• VEGA ID
  OTTHUMG00000002625

Gene info

• Identity
  HGNC:40203
• Gene
  ECE1-AS1
• Long gene name
  ECE1 antisense RNA 1
• Locus
  1p36.12
• Discovery year
  2019-07-25
• Entrez gene record
  100506801
• RefSeq identity
  • NR_038404
• Classification
  • Antisense RNAs

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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