ApoBrdU DNA Fragmentation Assay Kit
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Catalog number
K401-60
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Price
Please ask
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Size
60 assays
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Description
Detects DNA Fragmentation by FACS or FL
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Summary
• Detection method- Flow cytometry (Ex/Em = 488/520 nm for FITC, and 488/623 nm for PI) and fluorescence microscopy (FITC and rhodamine filters) • Species reactivity- Mammalian • Applications- The TUNEL-based assay kit provides complete components including positive and negative control cells for convenient detection of DNA fragmentation in cultured cells and tissue sections.
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Sample Type
Cell cultures (adherent and suspension), parafin embedded tissue sections
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Features Benefits
• Simple procedure; takes ~ 1 hour • Fast and convenient • The assay is sensitive and stable • The incorporation of BrdU in the procedure provides greater sensitivity than biotinylated or digoxigenylated methods.
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Storage Conditions
-20°C
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Shipping Conditions
gel pack
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Shelf life
12 months
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Background
Internucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells. BioVision’s Apo-BrdU In Situ DNA Fragmentation Assay Kit provides complete components including positive and negative control cells for conveniently detecting DNA fragmentation by fluorescence microscopy or flow cytometry. The kit utilizes Br-dUTP (bromolated deoxyuridine triphosphate nucleotides) which is more readily incorporated into DNA strand breaks than other larger ligands (e.g., fluorescein, biotin or digoxigenin). The greater incorporation gives rise to brighter signal when the Br-dUTP sites are identified by a fluorescein labeled anti-BrdU monoclonal antibody.
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Test
Biovision supplies other types of Assays as 1.
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Gene target
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Gene symbol
PRKDC, HLA-DOA
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Short name
ApoBrdU DNA Fragmentation Assay Kit
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Technique
Assay, dna, assays
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Host
Assay
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Alternative name
ApoBrdU Desoxyribonucleic acid Fragmentation test reagent
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Alternative technique
arrays, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
Gene info
Product images
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