Recombinant Human MAP4K5 / MEKKK5 Protein (His & GST tag)
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Catalog number
PKSH030349-20µg
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Price
Please ask
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Size
20µg
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Synonym
GCKR;KHS;KHS1;MAPKKKK5
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Activity
NA
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Sequence
Met 1-Tyr 846
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Fusion tag
N-His & GST
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Accession
NP_006566.2
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Expressed Host
Baculovirus-Insect Cells
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Shipping
Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.Shipment made at ambient temperature may seriously affect the activity of the ordered products.
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Purity
＞82 % as determined by SDS-PAGE
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Endotoxin
＜1.0 EU per µg as determined by the LAL method
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Stability and Storage
Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Mol Mass
123 kDa
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AP Mol Mass
116 kDa
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Formulation
Supplied as sterile 50mM Tris, 100mM NaCl, 0.5mM PMSF, 0.5mM TCEP, pH 8.5
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Background
Mitogen-activated protein kinase kinase kinase kinase 5, also known as Kinase homologous to SPS1/STE20, MAPK/ERK kinase kinase kinase 5, MEK kinase kinase 5 and MAP4K5, is a cytoplasm protein which belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and STE20 subfamily. MAP4K5 is ubiquitously expressed in all tissues examined, with high levels in the ovary, testis and prostate. It contains one CNH domain and one protein kinase domain. MAP4K5 is highly similar to yeast SPS1/STE20 kinase. Yeast SPS1/STE20 functions near the beginning of the MAP kinase signal cascades that is essential for yeast pheromone response. MAP4K5 has been shown to interact with CRKL and TRAF2. This kinase was shown to activate Jun kinase in mammalian cells. MAP4K5 is an early component of MAP kinase signal cascades. It may play a role in the response to environmental stress. MAP4K5 appears to act upstream of the JUN N-terminal pathway.
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Conjugation
histidine
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
MGST3, MAP4K5
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Short name
Recombinant MAP4K5 / MEKKK5 Protein (His & GST tag)
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
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Label
His
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Species
Human, Humans
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Alternative name
Rec. H. sapiens mitogen-activated protein kinase kinase kinase kinase 5 / MEKKK5 Protein (histidine & GST detection labelled)
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Alternative technique
rec
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Alternative to gene target
mitogen-activated protein kinase kinase kinase kinase 5, GCKR and KHS and KHS1 and MAPKKKK5, MAP4K5 and IDBG-6029 and ENSG00000012983 and 11183, transferase activity, Plasma membranes, Map4k5 and IDBG-144865 and ENSMUSG00000034761 and 399510, MAP4K5 and IDBG-646448 and ENSBTAG00000014792 and 781335
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Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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