This antibody has been succesfully tested for use in following methods:
Target of this antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified rabbit anti-human BMP-12 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA and 0.01% thimerosal.
Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Recombinant human BMP-12 (Cat.# 4572-20) can be used as positive control.
Shelf time: 12 months in temp.: -20 deg.C
This antibody reacts with:
Clonality of antibody:
E. coli expressed human BMP-12
How to handle this antibody:
The antibody solution should be gently mixed before use.
BMPs (bone morphogenetic proteins) belong to the TGF-β superfamily of structurally related signaling proteins. As implied by their name, BMPs promote and regulate bone development, growth, remodeling and repair, in both prenatal development and postnatal growth of eye, heart, kidney, skin, and other tissues. BMP-12 is highly conserved across species. BMP-12 regulates chondrogenesis, bone morphogenesis, and neuron differentiation.
Form of antibody:
Concentration of antibody:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.