Donkey anti-Rabbit IgG (H&L) - Affinity Pure, HRP Conjugate

• Catalog number
  MBS687579
• Price
  Please ask
• Size
  1 mg

• Products_type
  Secondary Antibody
• Products_short_name
  [IgG (H&L)]
• Products_name_syn
  [Donkey anti-Rabbit IgG (H&L) - Affinity Pure]
• Reactivity
  Rabbit. Cross Reactivity: Based on IEP, no reactivity is observed to non-immunoglobulin rabbit serum proteins
• Specificity
  Based on IEP, this antibody reacts with: heavy (gamma) chains on rabbit IgG, light chains on all rabbit immunoglobulins
• Purity
  Affinity purified antibody is > 95% based on SDS-PAGE Affinity purified using solid phase Rabbit IgG.
• Form
  Lyophilized
• Concentration
  1.0 mg/ml (E 1% at 280 nm = 13.0)
• Storage_stability
  Store freeze-dried powder at 2-8 degree C. Shelf Life: Store lyophilized material at 2-8 degree C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20 degree C as a liquid.
• Tested_application
  Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
• Test
  A high affinity purification column was use to purify anti-Rabbit IgG (H L) - Pure, Conjugate by MyBioSource by chromatographic size exclusion.
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. The ultrapure anti-Rabbit IgG (H L) - Pure, Conjugate supplied from MyBioSource is supplied in 1. The reagent contains very low traces of production synthesis impurities and is highly purified by ultra filtration and distillation for the chemical compounds.
• Latin name
  Equus africanus asinus, Oryctolagus cuniculus
• Properties
  HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
• Conjugation
  HRP Conjugated
• About
  Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rabbits are used for polyclonal antibody production by MyBioSource. Rabbit antibodies are very stable and can be stored for several days at room temperature. MyBioSource adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

• Gene target
  Conjugate  
• Short name
  anti-Rabbit IgG (H L) - Pure, Conjugate
• Technique
  affinity, Rabbit, anti-, anti, Pure, IgG, antibody to, antibodies, IgGs, pure products
• Host
  Donkey, Donkeys or Equus africanus asinus are often used as host species to raise antibodies. Even Equus ferus or horse antibodies are different than donkey antibodies since we know horse anti donkey IgGs. For immunohistochemistry it is useful to use an anti-donkey or horse block if your primary antibody is a donkey anti-human. We provide also anti-donkey as a species antibodies and ELISA kits. Rabbits
• Isotype
  IgG, Heavy & Light chains, IgG
• Label
  HRP
• Species
  Donkeys
• Alternative name
  Donkey Antibody tohost: rabbit Immunoglobulin G (heavy + light chain) - protein Pure, horseradish peroxidase Conjugate
• Alternative technique
  rabbit-anti, antibodies, purified, immunoglobulins

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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