AMPK⍺1 Antibody

• Catalog number
  3951-200
• Price
  Please ask
• Size
  200 µg

• Antibody Target
  AMPK⍺1
• Target Alternative Name
  5'-AMP-activated protein kinase, AMPK catalytic alpha-1 chain, AMPK alpha-1 chain
• Antibody Type
  Polyclonal
• Species Reactivities
  Human, mouse, rat
• Immunogen Sequence
  Synthetic peptide surroundign amino acid 486 of human AMPK alpha 1
• Accession
  NP_006242.5
• Gene Id
  5562
• Appearance
  Colorless liquid
• Form
  Liquid
• Concentration
  0.5 mg/ml
• Formulation
  200 µg (0.5 mg/ml) affinity purified rabbit anti-AMPKa1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal
• Purification
  Affinity purified
• Application
  Western blot
• Positive Control
  Jurkat cell lysate
• Application And Usages
  The antibody can be used for Western blotting (1–4 µg/ml).
• Description
  AMPK is responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. It appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. AMPK alpha1, a member of the Ser/Thr protein kinase family, is a catalytic subunit of AMPK.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• FACS Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  AMPK⍺1  
• Gene symbol
  PRKAA2
• Short name
  AMPK⍺1 Antibody
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  AMPK⍺1 (antibody to-)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:9377
• Gene
  PRKAA2
• Long gene name
  protein kinase AMP-activated catalytic subunit alpha 2
• Synonyms gene
  • PRKAA
• Synonyms gene name
  • protein kinase, AMP-activated, alpha 2 catalytic subunit
• Synonyms
  • AMPK
  • AMPKa2
• GenBank acession
  • BC069823
• Locus
  1p32.2
• Discovery year
  1995-12-12
• Entrez gene record
  5563
• Pubmed identfication
  • 7959015
• RefSeq identity
  • NM_006252
• VEGA ID
  OTTHUMG00000008282

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

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