Goat, IgM, affinity purified

  • Catalog number
    AS10 799
  • Price
    Please ask
  • Size
    2 mg
  • Available ordering format
    Liquid
  • Immunogen
    Purified human IgG
  • Raised in
    Goat
  • Clonality
    Polyclonal
  • Clone
    Polyclonal
  • Purification
    Affinity purified goat IgG
  • How to reconstitute
    See included datasheet or contact our support service
  • Storage condition
    Store undiluted liquid at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
  • Verified applications
    ELISA (ELISA)
  • Connected products
    other secondary antibodies to human immunoglobulins
  • Recommended dilutions for use
    needs to be determined by end user
  • Molecular weight expected аpparent
    see datasheet
  • Verified reactivity
    human IgG Fc (two heavy chains with constant domains)
  • Possible reactivity
    human IgG Fc (two heavy chains with constant domains),human IgG (H&L)
  • No reactivity
    no confirmed exceptions from predicted reactivity known in the moment
  • Supplementary information
    Antibody reacts with the heavy chains on human IgG based on immunoelectrophoresis.No reactivity is observed to light chains on human immunoglobulins and to human IgA and IgM based on immunoelectrophoresis.
  • References
    to be added when available
  • Scientific context
    Goat anti-human IgG Fc (two heavy chains with constant domains)is a secondary antibody which binds to human IgG Fc (two heavy chains with constant domains) in immunological assays.
  • Notes
    Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.05 % (w/v) of Sodium azide as preservative.Antibody concentration is 4.5 mg/ml.
  • Protein number
    Refer to NCBI
  • TAIR number
    Refer to NCBI
  • Test
    A high affinity purification column was use to purify Goat, IgM, by agisera by chromatographic size exclusion.
  • Description
    1 of protein A or G purified by agisera will give more specificity than crude serum.
  • Latin name
    Capra aegagrus hircus
  • Properties
    Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen
  • Short name
    Goat, IgM,
  • Technique
    affinity, purified, Goat, IgM, goats, IgMs, purifications
  • Host
    Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
  • Isotype
    IgM, IgM
  • Species
    Goats
  • Alternative name
    caprine, Immunoglobulin M, protein enriched
  • Alternative technique
    pure, caprine, immunoglobulin-m
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