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Available ordering format
Liquid
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Immunogen
Purified human IgG
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Raised in
Goat
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified goat IgG
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How to reconstitute
See included datasheet or contact our support service
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Storage condition
Store undiluted liquid at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
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Verified applications
ELISA (ELISA)
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Connected products
other secondary antibodies to human immunoglobulins
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Recommended dilutions for use
needs to be determined by end user
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Molecular weight expected аpparent
see datasheet
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Verified reactivity
human IgG Fc (two heavy chains with constant domains)
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Possible reactivity
human IgG Fc (two heavy chains with constant domains),human IgG (H&L)
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No reactivity
no confirmed exceptions from predicted reactivity known in the moment
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Supplementary information
Antibody reacts with the heavy chains on human IgG based on immunoelectrophoresis.No reactivity is observed to light chains on human immunoglobulins and to human IgA and IgM based on immunoelectrophoresis.
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References
to be added when available
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Scientific context
Goat anti-human IgG Fc (two heavy chains with constant domains)is a secondary antibody which binds to human IgG Fc (two heavy chains with constant domains) in immunological assays.
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Notes
Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.05 % (w/v) of Sodium azide as preservative.Antibody concentration is 4.5 mg/ml.
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Protein number
Refer to NCBI
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TAIR number
Refer to NCBI
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Test
A high affinity purification column was use to purify Goat, IgM, by agisera by chromatographic size exclusion.
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Description
1 of protein A or G purified by agisera will give more specificity than crude serum.
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Latin name
Capra aegagrus hircus
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Properties
Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen