Goat anti Human properdin

• Catalog number
  GAHu/PPD
• Price
  Please ask
• Size
  1 mL

• Category
  Primary Antibodies
• Long description
  The defined antibody specificity is restricted to properdin as tested at the level of sensitivity of immunoprecipitation techniques. A single precipitin line is obtained in immuno- electrophoresis, bidimensional electrophoresis and double radial immunodiffusion (Ouchterlony) against normal plasma, which shows a reaction of identity with the precipitated purified properdin. No reaction is obtained with any other plasma protein. In precipitating techniques as immunoelectrophoresis and single or double radial immunodiffusion. The presence of non-precipitating antibodies has not been assayed. This does not exclude the use of the antiserum in non-precipitating antibody-binding techniques if proper controls are included.
• Antibody come from
  Properdin is a factor of the alternative complement activation pathway. It has a molecular weight of 222,000 and consists of four peptide chains. Properdin is stabilizing the C3 convertase C3bBb. The mean concentration in plasma is 20 mg/ml. The antigen has been isolated form pooled human plasma. Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Other description
  Delipidated, heat inactivated, lyophilized, stable whole antiserum No preservative added. Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added.
• Clone
  Polyclonal
• Antigen antibody binding interaction
  Goat anti Human properdin Antibody
• Antibody is raised in
  Goat
• Antibody s reacts with
  Human
• Antibody s reacts with these species
  The antiSerum does not cross react with any other component of Human plasma. Inter- species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.
• Antibody s specificity
  Precipitating polyclonal Goat antiSerum to Human properdin
• Research interest
  Cardiovascular
• Application
  Immunoprecipitation
• Antibody s suited for
  Immunoprecipitation. In immunoelectrophoresis use 2 μl human serum against 120 μl antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl antiserum in a 3 mm diameter center well and 10 μl of the antiserum in 3 mm diameter peripheral wells. In single radial immunodiffusion use 1% antiserum in the gel. Precipitin titre 1:8 when tested against pooled normal human plasma in agar-block immunodiffusion titration.
• Storage
  The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day. Lyophilized at +4° C--at least 10 years. Reconstituted at or below -20° C--3-5 years. Reconstituted at +4° C--7 days
• Relevant references
  no information yet
• Protein number
  P27918
• Warnings
  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
• Antibody s link
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
• Latin name
  Capra aegagrus hircus
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

• Gene target
  properdin  
• Gene symbol
  CFP, CFB, CFD
• Short name
  Goat anti properdin
• Technique
  anti human, anti, Goat, antibody to, anti human antibodies, goats
• Host
  Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
• Isotype
  not specified
• Label
  unlabeled
• Species
  Human, Goats, Humans
• Alternative name
  caprine antibody to H. sapiens properdin
• Alternative technique
  antibodies, caprine

Gene info

• Identity
  HGNC:8864
• Gene
  CFP
• Long gene name
  complement factor properdin
• Synonyms gene
  • PFC
• Synonyms gene name
  • properdin P factor, complement
• GenBank acession
  • M83652
• Locus
  Xp11.23
• Discovery year
  1989-06-07
• Entrez gene record
  5199
• Pubmed identfication
  • 1783405
• RefSeq identity
  • NM_002621
• Classification
  • Complement system activation components
• VEGA ID
  OTTHUMG00000021451
• Locus Specific Databases
  • PFCbase: Mutation registry for properdin deficiency
  • Global Variome shared LOVD
  • LRG_129

Gene info

• Identity
  HGNC:1037
• Gene
  CFB
• Long gene name
  complement factor B
• Synonyms gene
  • BFD
  • BF
• Synonyms gene name
  • B-factor, properdin
• Synonyms
  • H2-Bf
• GenBank acession
  • L15702
• Locus
  6p21.33
• Discovery year
  1986-01-01
• Entrez gene record
  629
• RefSeq identity
  • NM_001710
• Classification
  • Complement system activation components
  • Sushi domain containing
• VEGA ID
  OTTHUMG00000031198
• Locus Specific Databases
  • LRG_136

Gene info

• Identity
  HGNC:2771
• Gene
  CFD
• Long gene name
  complement factor D
• Synonyms gene
  • DF
  • PFD
• Synonyms gene name
  • D component of complement (adipsin)
  • properdin factor D
  • complement factor D (adipsin)
• Synonyms
  • ADN
• Synonyms name
  • adipsin
• GenBank acession
  • M84526
• Locus
  19p13.3
• Discovery year
  1992-10-21
• Entrez gene record
  1675
• Pubmed identfication
  • 1374388
• RefSeq identity
  • NM_001928
• Classification
  • Granule associated serine proteases of immune defence
  • Complement system activation components
• VEGA ID
  OTTHUMG00000181840
• Locus Specific Databases
  • CFDbase: Mutation registry for Factor D deficiency (previously known as DFbase)
  • LRG_46

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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